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	1. Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus
	Qinghua Tian,Liqing Li,Jishuang Chen
	Biology 26 February 2009
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	Abstract:Two double-stranded RNAs were extracted from leaf tissues of Raphanus sativus-root cv. Yidianhong grown in Eastern China. Their full sequences, namely RasR7 and RasR8, were determined by a single primer amplification technique. Comparisons of 5′ untranslated regions (UTR) and 3′ UTR between RasR7 and RasR8 showed that they were highly conserved at 5′ UTR and an identical region 5′-AGAAUUU-3′ was discovered as the evidence of the same plant virus. 5′-UAAGAC-3′ was found as their identical region in 3′ UTR. BLASTP search in Genbank showed that RasR7 encoded putative protein was much similar with RNA dependent RNA polymerases (RdRps) of plant-infecting dsRNA viruses belonging to the family Partitiviridae. Phylogenetic analysis showed that RasR7 encoded putative protein clustered with the RdRps of members of the family Partitiviridae. RT-PCR detection indicated that both RasR7 and RasR8 existed in the virus-like particles. Therefore, it was suggested that RasR7 and RasR8 institute the genome of a newly discovered cryptic virus, namely Raphanus sativus cryptic virus 3 (RasV3).
	TO cite this article:Qinghua Tian,Liqing Li,Jishuang Chen. Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus [OL].[26 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29713


	2. Cross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii)
	Wang Ning,Chang Jiang,Sun Quanhui,Gu Langyu,Zhangzhengwang
	Biology 25 February 2009
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	Abstract:Cross-species microsatellite amplification can be an effective way in obtaining microsatellite loci for closely related taxa in bird species. The Reeves’s pheasant, Syrmaticus reevesii, is a vulnerable species endemic of China. In order to study the population genetics and paternity of its wild populations, we obtained 10 polymorphic microsatellite markers from cross-species amplification. The number of alleles per locus varies between two and twelve, with expected heterozygosity ranging from 0.32 to 0.802 (n =75). These polymorphic loci are facilitating the study of paternity identification of Reeves’s pheasant and also provide a valuable tool for future studies on conservation genetics of this species.
	TO cite this article:Wang Ning,Chang Jiang,Sun Quanhui, et al. Cross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii) [OL].[25 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29630

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	3. The study on hemo-compatibility of hydrophilic and hydrophobic SiCOH plasma coating
	WANG Gui-xue,ZHANG Qin,SHEN Yang,GE Shu-ping,JIA Dong-yu,TANG Chao-jun,YU Qing-song
	Biology 23 February 2009
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	Abstract:Low- temperature plasma deposition technique was used to prepare hydrophilic and hydrophobic SiCOH coating on the surface of silicon wafer. The flow ratio of two kinds of monomers, TMS and O2 could be controlled to obtain different hydrophilic plasma SiCOH coating. The results showed that, with increase of flow rate of O2, the hydrophilicity of surface with SiCOH coating could be improved, the water contact angle decreased from 105.8±3.0°to 29.8±2.1°. The results of blood proteins and platelets adhesion, activated partial thromboplastin time (aPTT) also showed that the blood-compatibility could be enhanced with the increase of hydrophilicity of plasma SiCOH coating.
	TO cite this article:WANG Gui-xue,ZHANG Qin,SHEN Yang, et al. The study on hemo-compatibility of hydrophilic and hydrophobic SiCOH plasma coating[OL].[23 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29506


	4. First Spiking Dynamics of Stochastic Neuronal Model with Optimal Control
	Wu Yongjun
	Biology 23 February 2009
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	Abstract:First-spiking dynamics of optimally controlled neuron under stimulation of colored noise is investigated. The stochastic averaging principle is utilized and the model equation is approximated by diffusion process and depicted by It?stochastic differential equation. The control problems for maximizing the resting probability and maximizing the time to first spike are constructed and the dynamical programming equations associated with the corresponding optimization problem are established. The optimal control law is determined. The corresponding backward Kolmogorov equation and Pontryagin equation are established and solved to yield the resting probability and the time to first spike. The analytical results are verified by Monte Carlo simulation. It has shown that the proposed control strategy can suppress the overactive neuronal firing activity and possesses potential application for some neural diseases treatment.
	TO cite this article:Wu Yongjun. First Spiking Dynamics of Stochastic Neuronal Model with Optimal Control[OL].[23 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29445


	5. Using Mammospheres as a Model to Determine Protein Signatures Presented in Breast Cancer Stem Cells
	guifa li,yuxiangjiang,dongchen,wiwang,yukuncui,fukunzhao
	Biology 23 February 2009
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	Abstract:The breast cancer stem cells have been proposed to underpin many types of breast Cancer. also,mammospheres have been widely employed to study breast cancer stem cells. In current study, we have used the approaches of Proteomics to study the altered protein expression in mammospheres from the most commonly used breast cancer cell line MCF-7. we have identified 34 differentially expressed proteins in Mammospheres from parental cells. Of these 34 proteins, only seven expressed higher in Mammosheres. We find that FKBP4 and GIPC1 which is high associated with insulin-like growth factor I receptor was low expressed in mammospheres. The two proteins and their implications in breast cancer drug resistance are currently under study in our laboratory.
	TO cite this article:guifa li,yuxiangjiang,dongchen, et al. Using Mammospheres as a Model to Determine Protein Signatures Presented in Breast Cancer Stem Cells[OL].[23 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29434


	6. Species difference in regional dispersal ability and neutral model
	Liu jiajia,Zhou ShuRong
	Biology 20 February 2009
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	Abstract:The neutral assumption is fundamental yet controversial to neutral theory. In real communities, species may differ in regional dispersal probability not only because of stochasticity such as random sampling process, but also the dispersal ability itself varies considerably across species. In this paper, we relaxed the ecological equivalence assumptions of the neutral model by introducing difference into species’ regional dispersal ability. Adding slight difference in dispersal ability among species into neutral model dramatically reduces species richness and result in considerable deviation of species abundance distributions from those predicted by neutral model. Difference in species immigration ability, if there is, can overwhelm the role of random drift and make local community dynamics becomes deterministic. Species with higher regional dispersal ability tends to have higher relative abundance in local communities. However, species relative abundance distribution curves under species differences in immigration rate can be fitted well by neutral model. More investigations are needed before we understand the relative roles of species differences and neutrality in structuring ecological communities.
	TO cite this article:Liu jiajia,Zhou ShuRong. Species difference in regional dispersal ability and neutral model[OL].[20 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29406


	7. The apical-basalateral polarization of RPE
	Jia Yanjuan,Song Dexiao,Liu Yong,Gao Lan
	Biology 18 February 2009
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	Abstract:Vertebrate retinal pigment epithelium (RPE) is a highly specialized epithelium with polarized membrane domains，with microvilli and junctional complexes on the apical pole and infoldings along the basolateral side, which express a morphological and functional polarity along their apical-to-basal axis. The loss of RPE polarity is associated with many degenerative eye diseases. Here, we highlight the polarized distribution of membrane protein and melanosome, TJs development, IPM and αPKC’s role and the alteration of RPE polarity in regeneration. Although RPE polarity has been studied a lot, which is at infant stage for treating degenerative eye diseases. So many morphological events and regulated mechanism still need to be elucidated in greater details from histological to molecular level, from development to adult, from nomal to mutation.
	TO cite this article:Jia Yanjuan,Song Dexiao,Liu Yong, et al. The apical-basalateral polarization of RPE[OL].[18 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29249


	8. A Filament-Assembling-Pressure Model for Developing Forces by Actin Polymerizing
	Wang Xiaoen
	Biology 13 February 2009
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	Abstract:The investigations of mechanisms for developing forces by actin polymerization come mainly from two models so far, sliding filament and Brownian ratchet models. Both models were difficult to clarify many experiment phenomena, and made some confusion at theory. Such mechanism is still not full understood because of difficulty in experiment observations at molecular level. Based on nucleation mechanisms of crystal, Young’s pressure, and ability of filaments to transmit water, this paper proposes a filament-assembling-pressure model by which can explain almost all of interrelated experiment phenomena. Assembly of a filament at its one end will produce redundant water because of monomer disappearance in the small microcosmic circumscription about polymerization site. The redundant water will diffuse to other end along the filament, and result in a pressure of water flow, filament-assembling pressure, by which propels loads, transfers signal or nutriments, and transforms chemical energy of polymerization into forces.
	TO cite this article:Wang Xiaoen. A Filament-Assembling-Pressure Model for Developing Forces by Actin Polymerizing[OL].[13 February 2009] http://en.paper.edu.cn/en_releasepaper/content/28983


	9. Transcriptional Profiling of Class A Rice Heat Stress Transcription Factors
	Ailing Liu,Xinbo Chen,Jie Zou,Xianwen Zhang,Xiaoyun Zhou,Wenfang Wang,Liyun Chen,Xinyao Xiong
	Biology 10 February 2009
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	Abstract:Expression profiles of twelve class A rice heat shock transcription factors genes (OsHsfA) were analyzed by semi-quantitative RT-PCR. The OsHsfA genes exhibited distinctive expressions in different organs and were affected differentially under abiotic stresses. Except for OsHsfA1a and OsHsfA3, all other OsHsfA family genes were strongly up-regulated under heat treatments; the majority of OsHsfA expression responses to salt, osmotic, and cold treatment primarily occur at the late stages of stress exposure. Our investigation implied that the twelve OsHSF genes may play different roles in plant development and abiotic stress responses.
	TO cite this article:Ailing Liu,Xinbo Chen,Jie Zou, et al. Transcriptional Profiling of Class A Rice Heat Stress Transcription Factors [J].


	10. Expression Profile of Young Rice Panicle under Heat Stress using DNA Microarray
	Xianwen Zhang,Ailing Liu,Jie Zou,Xiaoyun Zhou,Wenfang Wang,Liyun Chen,Xingyao Xiong,Xinbo Chen
	Biology 16 January 2009
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	Abstract:Heat shock has become one of the most serious stress factors to world crop yield and quality, and many genes responsive to heat stress and other abiotic and biotic stresses have been discovered and their functions have been elucidated. However, plant genome wide heat response mechanism still remains unclear. Here, we reported expression profile of young rice panicle under heat stress of 40°C on the whole genome level using rice 44k oligo-array. The results demonstrated that carbohydrate metabolism and stress-related genes consist of major group that are affected during the time course. Hsps, Hsfs, enzymes responsible for carbon partitioning, secondary metabolism, cell wall morphogenesis- related genes, and ETC are all sensitive to heat, and then the stress shed influence on DNA replication, and next on cell homeostasis, reproduction, and finally on cell cycle and photosynthesis. We tried to establish heat response model for rice panicle, which would be valuable to crop production and improvement.
	TO cite this article:Xianwen Zhang,Ailing Liu,Jie Zou, et al. Expression Profile of Young Rice Panicle under Heat Stress using DNA Microarray[J].

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