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1. A Fibre-Assembling-Pressure Model for Developing Root Pressure | |||
Wang Xiaoen | |||
Biology 12 December 2008 | |||
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Abstract:For decades, it has well been known that the drive force of conducting both water and dissolved mineral ions from roots to leaves of plants makes mainly up of two: the pull of transpiration from leaves and root pressure. It had early been clear with regard to the transpiring pull, but mechanisms how root pressure be developed, hitherto, is fuzzy. Based on both experimental phenomenon of siphon-like transport of water defying gravity along fibre surface and crystallisation mechanisms, this paper theoretically suggests a fibre-assembling-pressure model for developing root pressure. According to the model, in roots the continuous polymerization of fiberized network, such as formation of nascent cell wall and maturity of xylem, can develop the fibre-assembling pressure, a positive hydraulic pressure. For a plant that grows with vigor, growth of all of fibres, such as xylem, can provide a drive force for water transport, division and expansion of cells in meristems, and gravitropic growth of roots. | |||
TO cite this article:Wang Xiaoen . A Fibre-Assembling-Pressure Model for Developing Root Pressure [OL].[12 December 2008] http://en.paper.edu.cn/en_releasepaper/content/26564 |
2. Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L. | |||
Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong | |||
Biology 07 August 2008 | |||
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Abstract:More than seventy endophytic fungi were isolated by aseptic techniques from the phloem of the root of Ginkgo biloba L.. They were cultured in the potato dextrose (PD) liquid medium and one endophytic fungus (Gh01) was proved can produce orange pigment. The orange pigment was identified as quercetin glycoside by chemical reaction and HPLC. This is the first report on quercetin glycoside produced by endophytic fungus. The effects of the carbon and nitrogen sources, metal ions, initial pH and incubation temperature on pigment production of the endophytic fungus were evaluated. The optimal temperature and initial pH for pigment production in PD liquid medium were 28℃ and 7.0, respectively. Through the orthogonal trial, 20 g/L Glucose and 5 g/L peptone were the most suitable carbon and nitrogen source, 1 g/L chloridize zinc could increase the yield of pigment. Under the optimal conditions established, the maximum yield of the pigment was 27.515 g/L after 120 hours’ successive culture. | |||
TO cite this article:Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong. Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L.[OL].[ 7 August 2008] http://en.paper.edu.cn/en_releasepaper/content/23292 |
3. Antifungal activity of five solanaceous glycoalkaloids and their mixtures against phytopathogenic fungi Cercosporella brassicae and Alternaria porri | |||
ZHAO Xue-song,GAO Ling,WANG Juan,XU Wenjing,BI Hongtao,ZHOU Yi-fa | |||
Biology 22 July 2008 | |||
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Abstract:The antifungal activity of five solanaceae glycoalkaloids solanine, chaconine, solasonine, solamargine and tomatine against phytopathogenic fungi Cercosporella brassicae and Alternaria porri has been evaluated. Tomatine showed the highest antifungal activity against C. brassicae and A. porri among five compounds, followed with chaconie, solamargine and solasonine, while solanine showed the lowest antifungal activity. Mixture of potato glycoalkaloids solanine and chaconine produced marked synergistic antifungal activity. The magnitude of synergisms is higher at lower concentrations than that at higher concentrations. The antifungal activity of individual glycoalkaloid against A.porri was relative low, even no activity, but the mixture of chaconine and solanine show significant synergism. There was no synergism between glycoalkaloids solasonine and solamargine from Solanum nigrum in inhibiting fungul growth. The mixtures of solamargine and chaconine, and solasonine and solanine both caused additive inhibition on the growth of fungi. | |||
TO cite this article:ZHAO Xue-song,GAO Ling,WANG Juan, et al. Antifungal activity of five solanaceous glycoalkaloids and their mixtures against phytopathogenic fungi Cercosporella brassicae and Alternaria porri[OL].[22 July 2008] http://en.paper.edu.cn/en_releasepaper/content/22992 |
4. Molecular cloning and characterization of LeEBF1 and LeEBF2 from tomato | |||
Long Jiaoyue,Hu Zongli,Chen Xuqing,Yan Bo,Chen Guoping | |||
Biology 21 February 2008 | |||
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Abstract:Two full-length cDNAs of LeEBF1 and LeEBF2 were cloned from tomato (Solanum lycopersicum) by RT-PCR and rapid amplification of cDNA ends (RACE). They were 2866 and 2891 bp, with open reading frame (ORF) of 1911 bp and 1995 bp, encoding 637 and 665 amino acids, respectively. Putative LeEBF1 and LeEBF2 proteins, each containing a well-conserved F-box motif in the amino terminus and 14 tandem leucine-rich repeats (LRRs) in the carboxyl terminus, shared 58.6% of identity with each other. Identities with other species EBF proteins within F box motif ranged from 24.4% to 73.2%. RNA gel blot analysis results indicated that the expression levels of LeEBF1 and LeEBF2 in young leaves were higher than those in mature leaves of wild type and Nr. During the development of fruits, LeEBF1 and LeEBF2 expressed relatively weaker at IMG stage than at other stages experimented. Collectively, LeEBF1 and LeEBF2 may play an important role in leaf and fruit development of tomato. | |||
TO cite this article:Long Jiaoyue,Hu Zongli,Chen Xuqing, et al. Molecular cloning and characterization of LeEBF1 and LeEBF2 from tomato[OL].[21 February 2008] http://en.paper.edu.cn/en_releasepaper/content/18766 |
5. Testing the Hypothesis on Unidirectional Hybridization in Plants: Observations on Sonneratia, Bruguiera and Ligularia | |||
Renchao Zhou,Suhua Shi,David Boufford,ChungI Wu,Shi Suhua | |||
Biology 19 March 2007 | |||
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Abstract:When natural hybridization occurred at sites where the hybridizing species differ in abundance, the pollen load delivered to the rare species would consist predominantly of that from the common species. Previous authors have therefore proposed a hypothesis on the direction of hybridization: Interspecific hybrids are more likely to have the female parent from the rare species and the male parent from the common species. By examining the maternally inherited chloroplast DNA of six interspecific F1 hybrids from four genera of plants, we observed highly asymmetric hybridizations that appear to be inconsistent with the current hypothesis. Our results show that relative abundance of the hybridizing species is not a good predictor of the direction of hybridization. These unexpected observations may result from other mechanisms operating in the hybrid zones of plants. | |||
TO cite this article:Renchao Zhou,Suhua Shi,David Boufford, et al. Testing the Hypothesis on Unidirectional Hybridization in Plants: Observations on Sonneratia, Bruguiera and Ligularia [OL].[19 March 2007] http://en.paper.edu.cn/en_releasepaper/content/11515 |
6. Population genetic structure of three tree species in the mangrove genus Ceriops (Rhizophoraceae) from the Indo West Pacific | |||
Yelin Huang,Fengxiao Tan,Guohua Su,Shulin Deng,Suhua Shi | |||
Biology 19 March 2007 | |||
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Abstract:Ceriops is a viviparous mangrove with widespread species C. decandra and C. tagal, and the endemic species C. australis. Genetic diversity of the three species was screened in 31 populations collected from 22 locations in the Indo West Pacific using ISSR and sequences of partial nuclear gene (G3pdh) and chloroplast DNA (trnV-trnM). At the species level, the estimate of gene diversity (Ht) in C. decandra was 0.270, much higher than that in C. tagal (0.118) and C. australis (0.089) revealed by ISSRs. Heterozygous individuals were detected from one single population of C. tagal based on G3pdh sequences. Only C. decandra (four G3pdh haplotypes and three trnV-trnM haplotypes) was presented having more than one haplotype inferred from each DNA sequences data set. No haplotype diversity within population was detected in any of the three species, with the exception of the hybrid population. Three major geographical groups, correspond to the East Indian Ocean (EIO), South China Sea (SCS), and North Australia (NA) were identified in both C. decandra and C. tagal based on ISSRs. DNA sequences of C. decandra also detected the same three geographical groups by SAMOVA. Significant spatial genetic structure from populations between the three regions were detected, which may result from the historical geological events at these regions during the recent Pleistocene glaciations. This study also provided insights into the phylogenetics of Ceriops. | |||
TO cite this article:Yelin Huang,Fengxiao Tan,Guohua Su, et al. Population genetic structure of three tree species in the mangrove genus Ceriops (Rhizophoraceae) from the Indo West Pacific[OL].[19 March 2007] http://en.paper.edu.cn/en_releasepaper/content/11503 |
7. Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress | |||
Hong Yan | |||
Biology 20 September 2006 | |||
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Abstract:Several organic acids accumulated in Kochia Scoparia shoots and roots were studied by means of reverse-phrase high performance liquid chromatography with A C18 column. Five types of binary organic acids were separated. The organic acid concentrations were determined in K. Scoparia seedlings stressed by saline (NaCl) and alkaline (NaHCO3) at the same Na+ concentration. Concentrations of organic acids were stimulated by alkaline because the cells will adjust their pH values through the accumulation of organic acids, when the environment is basic. The concentrations of oxalic acid and succinic acid are higher than those of other organic acids, including tartaric acid and malic acid, and the concentration of citric acid is the lowest. The concentrations of organic acids in roots are higher than those in shoots under salt (NaCl) stress, but he results are opposite while the roots are under alkali (NaHCO3) stress. This indicates that there are different adaptive strategies for K. Scoparia seedlings in organic acid metabolism under salt and alkali stress | |||
TO cite this article:Hong Yan. Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress[OL].[20 September 2006] http://en.paper.edu.cn/en_releasepaper/content/8417 |
8. Identification of diurnal and circadian candidate genes in rice by using cDNA array | |||
Feng Zhang,Haitao Dong,Dean Jiang,Debao Li | |||
Biology 30 December 2005 | |||
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Abstract:cDNA array containing 5837 rice unique genes was used to explore gene expression patterns at 4 h intervals throughout a day. It was found that there were 190 genes with diurnal changes, of which 170 genes could change in circadian manner. Analysis of those genes showed that circadian clock played a key role in coordination of many physiological processes such as glycolysis, water and mineral elements transport. By clustering diurnal change genes from cDNA data, we found 10 clusters with different expression patterns. | |||
TO cite this article:Feng Zhang,Haitao Dong,Dean Jiang, et al. Identification of diurnal and circadian candidate genes in rice by using cDNA array[OL].[30 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4787 |
9. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan) | |||
Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong ,Feng Bi-Hong,Chen Yunfeng | |||
Biology 05 December 2005 | |||
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Abstract:Embryogenic callus induced from petioles of alfalfa (Medicago sativa L. cv. Jinnan) and maintained on kinetin (KN)-containing B5h medium becomes non-embryogenic with differentiation of tracheary elements when kinetin in the medium is replaced by thidiazuron (TDZ). To investigate the molecular mechanisms underlying the TDZ-induced morphological competence transition, we cloned the differentially expressed sequence tags (ESTs) from TDZ-treated callus of alfalfa using suppression subtractive hybridization (SSH). The expression patterns of genes induced by TDZ were found to change throughout the process of TDZ treatment. A homeodomain-leucine zipper protein gene, MSHB1, which was induced by TDZ, may be a transcription factor in the TDZ-regulation of alfalfa callus differentiation. The induction of trehalose-6-phosphate phosphatase (TPP), which may decrease the level of trehalose-6-phosphate, and the induction of 1-aminocyclopropane-1-carboxylate synthase (ACS), which increases the level of ethylene, by TDZ may be responsible for the termination of somatic embryogenesis. | |||
TO cite this article:Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong , et al. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan)[OL].[ 5 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4069 |
10. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence | |||
Tingting Qin,Jingfeng Fu,Nianhui Zhang,Linfang Du | |||
Biology 28 November 2005 | |||
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Abstract:The change patterns of senescence-related enzymes during cotyledon senescence were studied in a chlorophyll (Chl) b-deficient mutant type (MT) and its wild type (WT) of Brassica napus L. seedlings. The fresh weight on the basis of cotyledon number initially increased till 20 days after planting (DAP) and then kept relative constant. The protein content decreased sharply since 20 DAP and Chl content reduced since 10 DAP in both two types; however the rate of degradation in protein and Chl in the MT was slower than that in the WT since 20 DAP. Superoxide dismutase (SOD; E.C.1.15.1.1) activity declined in the WT but increased in the MT since 20 DAP. Activity of peroxidase (POD; E.C.1.11.1.7) increased markedly after 20 DAP in both types. Esterase (EST; E.C.3.1.1.1) activity increased in both two types since 10 DAP, whereas at 40 DAP it was much lower in the MT than that in the WT. In addition, bands patterns of SOD, POD and EST isozymes were changed during cotyledon development in both two types, but some differences were observed. Cu/ZnSODs activities were higher in the MT at 40 DAP as compared with the WT. These results showed that day 20 was the turning point during the cotyledon development and the senescence in Cr3529 cotyledon was slower than that in the WT. | |||
TO cite this article:Tingting Qin,Jingfeng Fu,Nianhui Zhang, et al. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence[OL].[28 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3804 |
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