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1. Analysis of gene expression in anther development of cytoplasmic male sterile Raphanas sativus L. | |||
SHI Shengli,WANG Jianbo | |||
Biology 20 January 2014 | |||
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Abstract:The anthers in ?owers perform important functions in sexual reproduction. Seldom studies have been made to study anther transcriptomes to explore genes controlling anther development. To analyze the characteristics of gene expression during the male sterile anther degeneration, we produced transcriptomes of anthers of radish (Raphanus sativus L.) at three progressive developmental stages with cDNA-AFLP method. The transcriptomes included at least 2556 unique transcripts, which involved in transcription, signal transduction, DNA synthesis, cell growth and others. The differential developmental pro?les of the transcripts signify extensive regulation of gene expression in the anther during development. The transcriptomes were used to dissect the major abnormality of gene expression of the sterile anther. First, we categorized and charted the developmental pro?les of all transcripts present in the anther; the transcripts of the sterile anther represent obviously difference contrast to the fertile anther. Second, we successfully selected genes from hundreds of transcripts encoding proteins for transcription, signal transduction, materials transport, and pollen wall formation. | |||
TO cite this article:SHI Shengli,WANG Jianbo. Analysis of gene expression in anther development of cytoplasmic male sterile Raphanas sativus L.[OL].[20 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4582792 |
2. Cloning and expression of a peroxiredoxin gene from Capsella bursa-pastoris | |||
TAO Peng,HUANG Xiaojun,WANG Jianbo | |||
Biology 14 January 2014 | |||
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Abstract:In this study, an antioxidant gene was isolated and cloned from the widespread plant Capsella bursa-pastoris. The antioxidant gene was homologous highly to the Arabidopsis ATPER1 gene, and was named as CbPER1. We deduced the amino acid sequence of CbPER1 protein, and used it to conduct a phylogenetic tree combining with 1-Cys Prxs (1-cysteine peroxiredoxins) from other plants for estimating the relationships. Using RT-PCR, we analyzed the expression of CbPER1 in different organs and embryonic stages. CbPER1 was extensively expressed in seeds, especially middle and later seeds. Besides, CbPER1 rarely responded to heat stress, cold stress, and ABA treatment in young leaves. Similarly, CbPER1 was not induced to express in young flowers when C. bursa-pastoris plants were treated with ABA. CbPER1 showed down-regulation in dormant embryos after GA treatment. Furthermore, the transcriptional level of CbPER1 in C. bursa-pastoris was compared to that of the Arabidopsis AtPER1, and both shared similar gene expression profiles. Our findings suggest that the CbPER1 genes play significant roles in seed maturation, dormancy, and germination, not in the response to abiotic stresses in C. bursa-pastoris. | |||
TO cite this article:TAO Peng,HUANG Xiaojun,WANG Jianbo. Cloning and expression of a peroxiredoxin gene from Capsella bursa-pastoris[OL].[14 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4582031 |
3. Phenotypic and growth variation of Spartina alterniflora along elevation gradient in saltmarsh on the coast of China | |||
Deng Zifa,Zhou Rong,Xie Xiaoling,Xie Huansong | |||
Biology 19 December 2013 | |||
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Abstract:Many researches demostrated there is a strong relationship between phenotypic plasticity and plant invasiveness, especially in a frequently disturbed environments. In order to reveal the phenotypic plasticity and reproductive traits of the invasive species, Spartina alterniflora, along an elevation gradient in the salt marsh of Jiangsu Province, Chian, five parallel sample zones were set from the seaward to the upland with a small scale elevation gradient, and the environmental factors, growth and photosynthetic parameters of Spartina alterniflora were surveyed. The results show that there are significant differences for four environmental factors (pH, salinity, organic matter and available nitrogen) and some growth parameters among five sample zones. The changes of salinity, organic matter and available nitrogen are in verse, and they show a bell-shaped. Plant phenotypic parameters show a similar variation as that of salinity along the elevation gradient. The sexual reproductive traits (the ratio of reproductive stem to vegetative stem, spike length, seed set and germination rate) in the seaward are significantly lower than that in other sample zones, however, the number of buds reproduced from rhizomes was the most among that of five zones. The rate of rhizome allocation (as an indicator of clonal reproduction) is highest, and the rate of spike allocation (an indicator of sexual reproduction) is the least in the seaward population among that of all zones. In addition, there is a positive feedback relationship between growth of S. alterniflora and soil nutrition (organic matter and total nitrogen). The results indicate that the invasive species S. alterniflora represents a strong phenotypic plasticity even though along a small elevation gradient in the saltmarsh, and this plasticity and the trade-off between sexual and clonal reproductive strategies may be of significant importance for the invasion and outbreak of S. alterniflora. | |||
TO cite this article:Deng Zifa,Zhou Rong,Xie Xiaoling, et al. Phenotypic and growth variation of Spartina alterniflora along elevation gradient in saltmarsh on the coast of China[OL].[19 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4576373 |
4. Identification of floral fragrances in freesia cultivars and hybrids by gas chromatography-mass spectrometry | |||
Xiao-yan Gao,Gao Xiang,Bao-feng Liu,Gao Fengzhan,Li Wang | |||
Biology 29 October 2013 | |||
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Abstract:Floral fragrance compounds of one freesia specie with four cultivars and ten hybrids were studied using static headspace extraction method followed by GC-MS analysis. In total, 43 compounds were identified with an average range of 3 and 25 compounds per plant. The main classes of compounds in most plants were isoprenoids, fatty acid derivatives accompanied by sulphur-containing compounds and nitrogen-containing compounds. Among the compounds, linalool (26.6-85.4%) was identified as the major component. In the parents and the studied progenies, the segregation laws of the scent traits in this cross were preliminarily unveiled and stable inheritance of the main components were observed. Based on the measurement of S?rensen's index of similarity (Is), nonmetric multidimensional scaling (MDS) was used to detect meaningful underlying dimensions and similarities between the investigated cultivars. The MDS analysis showed two groups of cultivars. These results were also discussed from the view of common pollinators like butterflies, moths, and bees. | |||
TO cite this article:Xiao-yan Gao,Gao Xiang,Bao-feng Liu, et al. Identification of floral fragrances in freesia cultivars and hybrids by gas chromatography-mass spectrometry[OL].[29 October 2013] http://en.paper.edu.cn/en_releasepaper/content/4566533 |
5. UV-irradiation and shade change the concentration of catalpol in Rehmannia glutinosa seedling and rhizosphere | |||
Zhang Peipei,Zhang Zhongtian,Gui Mengyuan,Wang Taixia | |||
Biology 25 June 2013 | |||
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Abstract:Research was conducted on Rehmannia glutinosa Libosch., a Chinese traditional medicinal plant, to investigate the effects of UV-irradiation and shade on the concentration of catalpol in seedlings and rhizosphere. The plants were grown under three irradiances: full light, partial shade (50% full light), and UV-irradiation. The R. glutinosa seedlings were harvested and the hydroponic culture solution in the containers was collected on day 3, 6, 9, 12, 15 after treatment respectively for determination of catalpol. The results showed that UV-irradiation increased the concentration of catalpol in leaves rapidly at the early stage, and then, instead of increasing, the concentration of catalpol decreased a little. In contrast, the catalpol content in R. glutinosa leaves was reduced under partial shade. Similar trends were observed in roots and stems of R. glutinosa under UV-irradiation and shade. The concentration of catapol in medium in which full-light, shade and UV-irradiated seedlings were grown all increased with time. But the concentration of catapol in medium of UV-irradiated seedlings was greater than that of full-light seedlings, and the concentration of catapol in medium of shade seedlings is lower. Therefore, UV-irradiation increased both production of catalpol in R. glutinosa seedlings, and secretion of catalpol into R. glutinosa rhizosphere. On the contrary shading decreased not only production of catalpol in R. glutinosa seedlings, but also secretion of catalpol into R. glutinosa rhizosphere. It was also found that UV-irradiation decreased the root vigor and partial shading increased the root vigor of R. glutinosa seedlings. | |||
TO cite this article:Zhang Peipei,Zhang Zhongtian,Gui Mengyuan, et al. UV-irradiation and shade change the concentration of catalpol in Rehmannia glutinosa seedling and rhizosphere[OL].[25 June 2013] http://en.paper.edu.cn/en_releasepaper/content/4548843 |
6. Analyzation of relationship between soil nutrients and podophyllotoxin accumulation in Podophyllum hexandrum and Dysosma versipellis | |||
LI Mengfei,ZHANG Jing,LI Jinghua,ZHOU Lanlan,LI Tiantian,LI Wei | |||
Biology 06 March 2013 | |||
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Abstract:In order to analyze the correlation between soil nutrients and PDT accumulation. The experiment was designed to determine the PDT content in rhizomes of P. hexandrum and D. versipellis by high performance liquid chromatography (HPLC) and nutrients content in soil by high performance ion chromatography (HPIC) and high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). The results of linear regress analysis showed that the PDT accumulation was positive relationship with NO3-, PO43-, Na+, Fe, Mn, and negative relationship with SO42-, K+, while Mg2+, Ca2+, Cu and Zn didn't reach significant levels. This study information can be helpful to produce PDT and related cyclolignans by commercial cultivation, cell and tissue cultures of P. hexandrum, D. versipellis and other related plant sources. | |||
TO cite this article:LI Mengfei,ZHANG Jing,LI Jinghua, et al. Analyzation of relationship between soil nutrients and podophyllotoxin accumulation in Podophyllum hexandrum and Dysosma versipellis[OL].[ 6 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4526883 |
7. Transcriptome analysis reveals genes involved in iridoid biosynthesis in Rehmannia glutinosa | |||
Sun Peng ,Lili Zhou,Xianen Li | |||
Biology 03 July 2012 | |||
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Abstract:Rehmannia glutinosa is a medicinal plant rich in iridoids, presently little is known about the iridoid biosynthesis in this plant. To explore the transcriptome of R. glutinosa and investigate genes involved in iridoid biosynthesis, we used massively parallel pyrosequencing on the 454 GS FLX Titanium platform to generate a substantial EST dataset. Based on sequence similarity searches against the public sequence databases, the sequences were annotated firstly and then were subjected to GO and KEGG based analysis. Bioinformatic analysis indicated that the 454 assembly contains a set of genes related to iridoid biosynthesis, including all genes involved in terpenoid backbone biosynthesis and four iridoid pathway genes that had been previously identified only in terpenoid indole alkaline producing plants, suggesting that iridods and secoiridoids share the common enzyme steps in the early stage of biosynthesis. In order to gain insight into how the isopentenyl diphosphate flux was controlled towards iridoid pathway, four prenyltransferase genes were analyzed using qRT-PCR and the results showed that the expression of the only geranyl diphosphate synthase is relative low in tuberous root, which indicated there may exist other enzymes responsible for the synthesis of geranyl diphosphate. | |||
TO cite this article:Sun Peng ,Lili Zhou,Xianen Li. Transcriptome analysis reveals genes involved in iridoid biosynthesis in Rehmannia glutinosa[OL].[ 3 July 2012] http://en.paper.edu.cn/en_releasepaper/content/4483757 |
8. Hypoglycemic and hypolipidemic effect of polyphenols from burs of Castanea mollissima Blume | |||
Yin Peipei,Zhao Shan,Siyu Chen,Jieyuan Liu,,Lingling Shi,Liu Yujun,Ma Chao | |||
Biology 26 March 2012 | |||
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Abstract:Substantial evidence suggests that phenolic extracts of Castanea mollissima Spiny burs(CMPE)increase pancreatic cell viability after STZ (streptozotocin) treatment as a result of their antioxidant properties. In the present study, the hypoglycemic and hypolipidemic activities of CMPE were studied in normal and STZ-induced diabetic rats. CMPE were orally administrated at dose of 150 and 300 mg/kg twice a day for 12 consecutive days. Serum glucose, triglyceride, total cholesterol, HDL- and LDL-cholesterol levels, malondialdehyde (MDA) level and SOD activity in liver, kidney, spleen and heart tissues were spectrophotometrically measured. In normal rats, no significant changes were observed in serum glucose, lipid profiles and tissue MDA and GSH levels after orally administration of CMPE. In diabetic rats, orally administration of CMPE at dose of 300 mg/kg caused significant decrease in serum glucose, triglyceride, total cholesterol, LDL-cholesterol levels, as well as MDA and GSH levels in spleen and liver tissues. However, dosage of 300 mg/kg caused a significant body weight lose in both normal and diabetic rats. The observed effects indicated that CMPE could be further developed as potential compounds to prevent abnormal changes in blood glucose and lipid profile and to attenuate lipid peroxidation in liver and spleen tissues. | |||
TO cite this article:Yin Peipei,Zhao Shan,Siyu Chen, et al. Hypoglycemic and hypolipidemic effect of polyphenols from burs of Castanea mollissima Blume[OL].[26 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4473103 |
9. Transcriptome sequencing of a highly salt tolerant mangrove species Sonneratia alba using Illumina platform | |||
Zhou Renchao ,Sufang Chen | |||
Biology 19 March 2012 | |||
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Abstract:Mangroves are critical and threatened marine resources, yet few transcriptomic and genomic data are available in public databases. The transcriptome of a highly salt tolerant mangrove species, Sonneratia alba, was sequenced using the Illumina Genome Analyzer in this study. Over 15 million 75-bp paired-end reads were assembled into 30,628 unique sequences with an average length of 581 bp. Of them, 2,358 SSRs were detected, with di-nucleotide repeats (59.2%) and tri-nucleotide repeats (37.7%) being the most common. Analysis of codon usage bias based on 20, 945 coding sequences indicated that genes of S. alba were less biased than those of some microorganisms and Drosophila and that codon usage variation in S. alba was due primarily to compositional mutation bias, while translational selection has a relatively weak effect. Genome-wide gene ontology (GO) assignments showed that S. alba shared a similar GO slim classification with Arabidopsis thaliana. High percentages of sequences assigned to GO slim category 'mitochondrion' and four KEGG pathways, such as carbohydrates and secondary metabolites metabolism, may contribute to salt adaptation of S. alba. In addition, 1,266 unique sequences matched to 273 known salt responsive genes (gene families) in other species were screened as candidates for salt tolerance of S. alba, and some of these genes showed fairly high coverage depth. At last, we identified four genes with signals of strong diversifying selection (Ka/Ks >1) by comparing the transcriptome sequences of S. alba with 249 known ESTs from its congener S. caseolaris. This study demonstrated a successful application of the Illumina platform to de novo assembly of the transcriptome of a non-model organism. Abundant SSR markers, salt responsive genes and four genes with signature of natural selection obtained from S. alba provide abundant sequence sources for future genetic diversity, salt adaptation and speciation studies. | |||
TO cite this article:Zhou Renchao ,Sufang Chen. Transcriptome sequencing of a highly salt tolerant mangrove species Sonneratia alba using Illumina platform[OL].[19 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4469396 |
10. Sonneratia ovata Backer - A genetically depauperate mangrove species | |||
Zhou Renchao*,Suo Qiu | |||
Biology 19 March 2012 | |||
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Abstract:It is expected that geographically widespread and outcrossing tree species would have high level of genetic variation. Three chloroplast regions and six nuclear genes were sequenced to assess the genetic variation of a species of mangroves, Sonneratia ovata, from China and Thailand. No nucleotide polymorphism was found in the three chloroplast regions and in the six nuclear genes from all of the four populations examined. The depauperation of polymorphism in S. ovata in comparison to moderate polymorphisms of other congeneric species is surprising, particularly considering high level of polymorphism in the past and relatively wide geographic distribution of the species. Since multiple independent loci were surveyed in this study, the most plausible explanation for our observation is that S. ovata has experienced severe demographic bottlenecks in the Pleistocene glaciation, followed by subsequent recolonization by sea currents in China and Southeast Asia regions. The lack of polymorphism may also be attributable to its small population size, despite its wide geographic distribution. | |||
TO cite this article:Zhou Renchao*,Suo Qiu. Sonneratia ovata Backer - A genetically depauperate mangrove species[OL].[19 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4469332 |
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