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1. Sorption, desorption and mobility characteristics of a novel fungicide pyrazoxystrobin in three agricultural soils | |||
Liu Xunyue,Ding Xingcheng | |||
Agronomy 20 January 2012 | |||
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Abstract:Pyrazoxystrobin, (E)-methyl 2-(3-((3-(4-chlorophenyl)-1-methyl- 1H -pyrazol-5-yloxy) methyl) phenyl) -3 - methoxyacrylate, is a novel fungicide developed in China. The adsorption, desorption and mobility characteristics of pyrazoxystrobin on three agricultural soils were studied by using 14C labeled compound. The adsorption of pyrazoxystrobin in soils was correlated to soil organic matter content and soil pH and the sorption has been observed in the order of S2>S1>>S3. Desorption was reflect some of the interactions involved between the pesticides and the soil components, soil pH seems to more influence to pyrazoxystrobin desorption and only about 2.54 to 6.41% of adsorbed pyrazoxystrobin was released. The mobility result, minimum of 95.02% compound residued in the upper 4.0 cm layer, demonstrated the use of pyrazoxystrobin as a fungicide is likely to be safe to groundwater. | |||
TO cite this article:Liu Xunyue,Ding Xingcheng. Sorption, desorption and mobility characteristics of a novel fungicide pyrazoxystrobin in three agricultural soils[OL].[20 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463029 |
2. The impact of environmental heterogeneity on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae) | |||
HUANG Shengwei,ZHANG Hongyu | |||
Agronomy 11 January 2012 | |||
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Abstract:In this paper, we demonstrated the composition and diversity of microbial communities in hindgut of Holotrichia parallela larvae, and investigate how did intestine microbial communities respond to environmental heterogeneity. Methods: Extract total DNA of gut bacteria, construct clone libraries of 16S rDNA, and DGGE was performed to analyze the clone libraries. Sequencing and phylogenetic analysis were performed to determine the difference of intestine microbial communities among different instars. Results: Firmicutes and Proteobacteria were the major phyla of the hindgut microbiota. Bacterial diversity (Chao1 and Shannon indices) and community structure varied across the sampled populations, and the observed variation can be explained by environmental parameters of the locations where the populations were sampled. Conclusions: Our results suggest that the gut's bacterial community changes in response to environmental heterogeneity, possibly to meet the host's ecological needs or physiological demands. | |||
TO cite this article:HUANG Shengwei,ZHANG Hongyu. The impact of environmental heterogeneity on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae)[OL].[11 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4461358 |
3. The impact of life stage on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae) | |||
HUANG Shengwei,ZHANG Hongyu | |||
Agronomy 11 January 2012 | |||
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Abstract:In this paper, we demonstrated the composition and diversity of microbial communities in hindgut of Holotrichia parallela larvae, and investigate how did intestine microbial communities respond to life stages. Methods: Extract total DNA of gut bacteria, construct clone libraries of 16S rDNA, and DGGE was performed to analyze the clone libraries. Sequencing and phylogenetic analysis were performed to determine the difference of intestine microbial communities among different instars. Results: Increases in the species richness and diversity of gut microbiota were observed during larval growth. Bacteroidetes comprised the dominant group in the first instar; however, Firmicutes composed the majority of the hindgut microbiota during the second and third instars. Conclusions: Our results suggestted that the gut's bacterial community changes in response to the host's physiology, possibly to meet the host's ecological needs or physiological demands. | |||
TO cite this article:HUANG Shengwei,ZHANG Hongyu. The impact of life stage on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae)[OL].[11 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4461355 |
4. Comparative population genetics of Liposcelis bostrychophila and L. entomophila, from China inferred by analysis of the mitochondrial Cytb Gene | |||
WEI Dandan,WU Jingjing,DOU Wei,WANG Jinjun | |||
Agronomy 10 January 2012 | |||
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Abstract:The psocids Liposcelis bostrychophila and L. entomophila (Psocoptera: Liposcelididae), are two major pests of stored grain and commonly occur on a wide range of stored products. Increasingly, psocids have gained recognition of their importance due to their feeding on stored grains, contaminating food and agricultural commodities. Psocids are difficult to control and exhibit high levels of resistance to various insecticides. Previous research has mainly focused on psocid physiology, biochemistry, and basic biology in grain storage systems. However, the population genetic structure has not been well categorized, which may be useful for understanding the distribution of resistance gene frequencies among psocid populations, gene flow patterns, dispersal of source populations, and the resultant genetic structure among populations. We generated mtDNA sequence data for 148 L. entomophila individuals from ten collecting locations and 103 L. bostrychophila individuals from eight locations in China. Population genetic analyses indicated that the obligatory parthenogenetic L. bostrychophila had high genetic diversity, and is higher than the genetic diversity of sexually reproducing psocid L. entomophila. Both species had a significant genetic structure and significant genetic differentiation existed amongst the populations. The Mantel test indicated that there was no evidence for isolation-by-distance for both species. The neutrality test revealed that the two species might have undergone population expansions in the past in the sampled areas. In addition, population differentiation of both psocids may mainly due to other factors such as genetic drift or inbreeding and less by geographic distance. | |||
TO cite this article:WEI Dandan,WU Jingjing,DOU Wei, et al. Comparative population genetics of Liposcelis bostrychophila and L. entomophila, from China inferred by analysis of the mitochondrial Cytb Gene[OL].[10 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4461243 |
5. Identification of HrpX Regulon Genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique | |||
LI Yurong,XIAO Youlun,ZOU Lifang,ZOU Huasong,CHEN Gongyou | |||
Agronomy 02 November 2011 | |||
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Abstract:Xanthomonas oryzae pv. oryzicola is the causal agent of bacterial leaf streak in rice and injects repertoires of T3S effectors (T3SEs), which are normally regulated by a global regulator HrpX, into plant cells to suppress plant innate immunity for disease development. To establish a visualization technique to identify HrpX regulon genes in this pathogen, we chose six known or unknown T3SE genes of X. oryzae pv. oryzicola (strain RS105) as the targets in this report. The promoters of these candidates, whether or not containing a PIP-box (plant-inducible promoter), were fused with the gfp (green fluorescent protein) reporter gene, and the gfp reporters were introduced into the wild-type RS105 and the hrpX mutant RΔhrpX. GFP expression in the wild-type strain could be observed under fluorescence microscopy, but fluorescence was not observed in the hrpX mutant. Consequently, transcription and secretion detection demonstrated that these HrpX regulon members were repressed when hrpX was mutated and were not secreted into the hrp-inducing medium XOM3 because of a mutation in hrcV (T3SS deficient). These data suggest that the gfp reporter system is a feasible visualization tool for identification of HrpX regulon genes and discovery of new T3SEs in the X. oryzae pv. oryzicola-rice pathosystem. | |||
TO cite this article:LI Yurong,XIAO Youlun,ZOU Lifang, et al. Identification of HrpX Regulon Genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique[OL].[ 2 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4447535 |
6. Biosuppression of fungal decay in pear fruit by chitosan integration with biocontrol yeast and calcium chloride | |||
YU Ting,LU Huangping,ZHENG Xiaodong | |||
Agronomy 02 November 2011 | |||
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Abstract:This study evaluated the performance of chitosan at different concentrations alone, and in combination with a biocontrol yeast Cryptococcus laurentii and calcium chloride in control of the blue mold decay in pear fruit. The results showed that efficacy of chitosan in inhibiting blue mold decay in pear fruit decreased as the incubation time increased. Chitosan at 0.5% or above delayed the rapid growth of C. laurentii, nevertheless, it enhanced remarkably mold decay control when in combination with C. laurentii. Moreover, addition of calcium chloride the mixture of C. laurentii and chitosan further enhanced the decay control, although calcium chloride had little antifungal activity. Therefore, a combination of chitosan with C. laurentii and calcium chloride provides a great new potential for development of effective alternative approach to inhibit the blue mold decay especially in an integrated postharvest diseases management strategy. | |||
TO cite this article:YU Ting,LU Huangping,ZHENG Xiaodong. Biosuppression of fungal decay in pear fruit by chitosan integration with biocontrol yeast and calcium chloride[OL].[ 2 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4448268 |
7. Effect of Cryptococcus laurentii and calcium chloride on reduction of Penicillium expansum and Botrytis cinerea infections in pear fruit | |||
YU Ting,LU Huangping,ZHENG Xiaodong | |||
Agronomy 26 October 2011 | |||
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Abstract:This study was conducted to evaluate the efficacy of the biocontrol yeast Cryptococcus laurentii and calcium chloride (CaCl2) in suppressing the blue and gray mold rots of pear and to explore possible mode of action involved. The results showed that the combined treatment of pear fruit wounds with C. laurentii and CaCl2 at 2% (w/v) resulted in a significantly improved inhibition of Penicillium expansum and Botrytis cinerea infections in comparison with the application of C. laurentii alone. CaCl2 neither affected the growth of C. laurentii in vitro or in vivo, nor directly inhibited the blue mold in pear fruit wounds, however, it induced the fruit resistance to the blue mold rot when the time interval between CaCl2-treatment and pathogen-inoculation was up to 24 h, being associated with an activation of the peroxidase activity of pear fruit. Therefore, it could be concluded that the mechanism by which CaCl2 reinforced the biocontrol efficacy of C. laurentii against the mold infections was mainly due to its ability to inducing the fruit natural resistance. | |||
TO cite this article:YU Ting,LU Huangping,ZHENG Xiaodong. Effect of Cryptococcus laurentii and calcium chloride on reduction of Penicillium expansum and Botrytis cinerea infections in pear fruit[OL].[26 October 2011] http://en.paper.edu.cn/en_releasepaper/content/4447456 |
8. Molecular analysis of quizalofop-resistant Echinochloa crusgalli reveals a Leu-1781-Ile mutation | |||
HUAN Zhibo,WANG Jinxin | |||
Agronomy 06 March 2011 | |||
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Abstract:A resistant Echinochloa crusgalli (barnyard grass) population which survived after repeated application of quizalofop in China was collected. After self-pollination and outcross, a homozygous germplasm (RR) was obtained. The resistant germplasm (RR) was found highly resistant to quizalofop compared with the sensitive germplasm (SS) with the resistance ratio 86.1. In order to determine the mechanism of this resistance, the chloroplastic ACCase cDNA from RR and SS were cloned. After compared the deduced amino acid sequences, a single isoleucine-leucine substitution at position 1781 (position number standardized to Alopecurus myosuroides (black grass)) was found. This mutation was identical with the formerly reported results in R population of other weed species. In the allele-specific PCR assay, we found RR and SS were all homozygous. All the copies in RR were Leu1781 and SS were Ile1781.????? | |||
TO cite this article:HUAN Zhibo,WANG Jinxin. Molecular analysis of quizalofop-resistant Echinochloa crusgalli reveals a Leu-1781-Ile mutation[OL].[ 6 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4414727 |
9. Effect of vegetation of transgenic Bt rice and its straw amendment on soil enzymes, respiration and functional diversity of soil microorganisms under field conditions | |||
Dong Bin,Yan Hu,Yu Yunlong | |||
Agronomy 17 December 2010 | |||
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Abstract:With the development of transgenic crops, there is an increasing concern about the possible adverse effects of their vegetation and residues on soil environmental quality. To evaluate the effect of vegetation of transgenic Bt rice followed by return of rice straw on microbe-mediated functions in soil under field conditions, two Bt transgenic rice lines, Huachi B6 (Bt-HC) and TT51 (Bt-T), were adopted to assess the possible effect on soil enzyme (neutral phosphatase, urease, catalase, and invertase) activities, anaerobic respiration activity, and microbial utilization of carbon substrates. The results indicated that the vegetation of the two transgenic rice lines, Huachi B6 (Bt-HC) and TT51 (Bt-T), and addition of their straw have few adverse effect on soil enzymes and anaerobic respiration activity, compared to their parent and distant parent, although some transient or even significant differences were observed. The vegetation and straw amendment of Bt rice B6 (Bt-HC) and TT51 (Bt-T) did not appear to have harmful effect on the richness and evenness of soil microorganisms. The dominant population of soil microorganisms seemed to be more sensitive and altered temporarily by vegetation and straw amendment of Bt rice. This alteration is, however, smaller than that caused by vegetation and straw amendment of non-transgenic distant parental rice. No different pattern of impact duo to plant species was found between B6 (Bt-HC) and TT51 (Bt-T). It could be concluded from the results obtained in this study that vegetation of Bt rice and the return of its straw as fertilizer will not alter soil microbe-mediated functions. | |||
TO cite this article:Dong Bin,Yan Hu,Yu Yunlong. Effect of vegetation of transgenic Bt rice and its straw amendment on soil enzymes, respiration and functional diversity of soil microorganisms under field conditions[OL].[17 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4398651 |
10. Dissipation of carbendazim and chloramphenicol alone and in combination, and their effects on soil fungi:bacteria and soil enzyme activities | |||
Yan Hu,Dong Bin,Yu Yunlong | |||
Agronomy 17 December 2010 | |||
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Abstract:The dissipation of carbendazim and chloramphenicol alone and in combination, and their effects on soil fungi:bacteria and soil enzyme activities were investigated. The results revealed that carbendazim dissipation was little affected by chloramphenicol, whereas chloramphenicol dissipation was found to be retarded significantly by the presence of carbendazim. The inhibitory effect of carbendazim on fungal:bacterial ratio and chloramphenicol on neutral phosphatase was enlarged by the presence of chloramphenicol and carbendazim, respectively. The increase in soil catalase and urease activities caused by carbendazim was partially eliminated by the presence of chloramphenicol. Little interaction between carbendazim and chloramphenicol was observed for their influence on soil invertase. The results obtained in this study suggested that combination of fungicide and antibiotic may alter their individual behavior in soil, and their effects on soil enzymes. | |||
TO cite this article:Yan Hu,Dong Bin,Yu Yunlong. Dissipation of carbendazim and chloramphenicol alone and in combination, and their effects on soil fungi:bacteria and soil enzyme activities[OL].[17 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4398636 |
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