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| There are 181 papers published in subject: since this site started. | |||
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| 1. L-Ficolin Binds to the Glycoproteins Hemagglutinin and Neuraminidase and Inhibits Influenza A Virus Infection Both in vitro and in vivo | |||
| PAN Qin,WANG Feng,CHEN Hadan,HOU Wei,Victor Tunje Jeza,ZHAO Yinglan,ZHANG Xiao-Lian | |||
Basic Medicine 21 March 2013
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| Abstract:L-ficolin, one of the complement lectins found in human serum, is a novel pattern recognition molecule that can specifically bind to microbial carbohydrates, thereby activating the lectin complement pathway and mounting a protective innate immune response. However, little is known about the role of L-ficolin during viral infections in vivo. In the present study, we used a mouse model of influenza A virus infection to demonstrate that the administration of exogenous L-ficolin or ficolin A (FCNA - an L-ficolin-like molecule in the mouse) is protective against the virus. Furthermore, FCNA-null mice have a greatly increased susceptibility to infection with the influenza A virus. Moreover, we found recombinant human L-ficolin inhibited influenza A virus entry into Madin-Darby canine kidney cells. More importantly, L-ficolin can recognize and bind hemagglutinin (HA) and neuraminidase (NA) glycoproteins and different subtypes of influenza A virus,and these interactions can be competitively inhibited by N-acetyl-D-glucosamine. In addition, the binding of L-ficolin and FCNA may lead to the activation of the lectin complem e nt p athw ay. To o ur k n ow l e d g e, this is th e f ir s t re p o r t d e m onstrating that L-ficolin can bl ock influenza virus infections both in vitro and in vivo using FCNA-knockout mice, possibly by interacting with the carbohydrates of HA and NA. Therefore, these data may provide new immunotherapeutic strategies based on the innate immune molecule L-ficolin against the influenza A virus. | |||
| TO cite this article:PAN Qin,WANG Feng,CHEN Hadan, et al. L-Ficolin Binds to the Glycoproteins Hemagglutinin and Neuraminidase and Inhibits Influenza A Virus Infection Both in vitro and in vivo[OL].[21 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4531323 | |||
| 2. The Adsorption and Release of DNA by Mesoporous Silica Materials with Different Pore Diameters. | |||
| SUN Yan,YAN Tingsheng,LIU Xianbin | |||
| Basic Medicine 18 March 2013 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Safe and efficient vector systems played a crucial role in the transgenic technology. Mesoporous silica (MS) materials, which can be modified with functional groups, have the potential of being used as good vectors. In this study, the pore diameters of synthetic materials were from 2.3 nm to 5.0 nm and the samples have a good dispersion. The MS meterials with different pore diameters were successfully amino-modified and studies about the adsorption and release properties of DNA were carried out. The sample N-MCM-41-14 which had the smallest pore 2.3 nm could only adsorb DNA fragment shorter than 750 bp. The sample N-SBA-15 with 5.0 nm pore could adsorb all DNA fragment shorter than 5000 bp. The pore size of MS materials had important implications on the DNA adsorption rate and the maximum adsorption capacity. However, pore size of the materials had little effect on the release rate of DNA. In sum, MS materials with different pore diameters had different adsorption properties of DNA, and could be as prefect gene delivery systems. | |||
| TO cite this article:SUN Yan,YAN Tingsheng,LIU Xianbin. The Adsorption and Release of DNA by Mesoporous Silica Materials with Different Pore Diameters.[OL].[18 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4530263 | |||
| 3. Case-control study of FGF20 polymorphism in sporadic Parkinson's disease in Northern Han Chinese | |||
| XU Xiaofeng,XU Huamin,XIE Anmu,JIANG Hong,XIE Junxia | |||
| Basic Medicine 07 March 2013 | |||
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| Abstract:Fibroblast growth factor 20 (FGF20) is a neurotrophic factor that exerts neurotrophic properties in the brain and could significantly enhance the survival of rat midbrain dopaminergic neurons. The genetic association of the FGF20 gene with Parkinson's disease (PD) remains controversial. Herein we used polymerase chain reaction-restriction length polymorphism assay to assess the association of two single nucleotide polymorphisms (SNPs) rs12720208 and rs1721100 within FGF20 gene in 178 PD patients and 190 healthy controls in Northern Han Chinese. Results showed no significant differences in the rs1721100 or rs12720208 of FGF20 gene between PD cases and the controls. This indicates FGF20 gene might not play a major role in the genetic predisposition to PD in this population. | |||
| TO cite this article:XU Xiaofeng,XU Huamin,XIE Anmu, et al. Case-control study of FGF20 polymorphism in sporadic Parkinson's disease in Northern Han Chinese[OL].[ 7 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4527523 | |||
| 4. 6-Hydroxydopamine Promotes Iron Traffic in Primary Cultured Astrocytes | |||
| ZHANG Haoyun,SONG Ning,XU Huamin,SHI Limin,JIANG Hong,XIE Junxia | |||
| Basic Medicine 06 March 2013 | |||
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| Abstract:It is well known that disrupted brain iron homeostasis was involved in Parkinson's disease (PD). Astrocytes, the major glial cell type in the central nervous system, are largely responsible for iron distribution in the brain. However, how iron metabolism changes of astrocytes in PD are not fully elucidated. In the present study, we first observed that both iron influx and efflux were enhanced with 10 μM 6-OHDA treatment for 24 hrs in primary cultured astrocytes. In accordance with this iron traffic modulations, iron importer divalent metal transporter 1 with iron responsive element (DMT1+IRE) and exporter ferroportin 1 (FPN1) were up-regulated in these cells. Iron regulatory protein 1 (IRP1) showed a dynamic regulation with 6-OHDA treatment, as indicated by a moderate up-regulation at 12 hrs, however, down-regulation at 24 hrs. These results suggest that 6-OHDA might promote iron transport rate in astocytes by regulating iron transporters and IRP1 expression. | |||
| TO cite this article:ZHANG Haoyun,SONG Ning,XU Huamin, et al. 6-Hydroxydopamine Promotes Iron Traffic in Primary Cultured Astrocytes[OL].[ 6 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4527136 | |||
| 5. N-glycan-defective breast cancer cellsinduce a phenotypic switch inpolarization of bone marrow-derivedmacrophages | |||
| Chen Haidan ,huili CAI,LiDongqing | |||
| Basic Medicine 27 February 2013 | |||
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| Abstract:Purpose: To investigate the e!ect of N-glycan-defective mammary adenocarcinoma cells on the polarization of macrophages. Methods: N-glycan-defective breast cancer cells (MA782 cells) were prepared by swainsonine(SW) treatment and the cytotoxicity of SW to MA782 cells was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. "e Nglycan-defective MA782 cells were co-cultured with bone marrow-derived macrophages (BMDMs) for 48 h in vitro, and then the BMDMs and the co-cultured supernatant were analyzed for macrophage phenotypic using FQRT-PCR, FCM and ELISA. Results: SW-treated MA782 cells expressed defective N-glycan on the cell surface in a dose-dependent manner (*p<0.05). MTT assays showed that neither the 1 ug/mL nor 5ug/ mL SW treatments showed signi$cant inhibition of MA782 cell growth in vitro. "e expression of iNOS and agr-1 in the 5 #g/mL SW-treated group were 4.75-fold higher and 3.7-fold lower than that in the untreated group, respectively (*p<0.05). Mean %uorescence intensity of CD16/32 expressed in the cells treated with 5 #g/mL SW was signi$cantly higher in comparison with the untreated group (65 vs. 7, *p<0.05), though the percentage of CD16/32-positive cells were not signi$cantly di!erent. Furthermore, the expression of CD206 and dectin-1 in the 5 #g/mL SW-treated group was signi$cantly decreased (3.1±0.3% and 4.1±1.1%, respectively) in comparison with the untreated group (40±3% and 8.9±1.2%, respectively, both p<0.05). In addition, the 5 #g/mL SW-treated group secreted more TNF-alpha (350 ±25 pg/mL) and less IL-10 (89±7.2 pg/mL) than the untreated group (80 ±3 pg/mL and 150 ±10 pg/mL, respectively, both p<0.05). Conclusion: N-glycan-defective MA782 cells can induce the di!erentiation of BMDM into proin%ammatory M1 macrophages in vitro. | |||
| TO cite this article:Chen Haidan ,huili CAI,LiDongqing. N-glycan-defective breast cancer cellsinduce a phenotypic switch inpolarization of bone marrow-derivedmacrophages[OL].[27 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4522243 | |||
| 6. The Cell Uptake of Mesoporous Silica Nanoparticles Carrying DNA in Vitro | |||
| SUN Yan,LIN Li,LIU Xianbin | |||
| Basic Medicine 25 February 2013 | |||
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| Abstract:The application of mesoporous silica nanoparticles (MSN) in gene carrying get more and more researchers' attention with its superior properties. In this paper, we researched the two kinds of cancer cell uptake of the MSNs that carried different DNA. The small molecule nucleic acid can successfully access into the interal pore of the nanoparticles with large quantitives, and the MSNs also play a good protection effect on the DNA absorbed. Interestingly, the more quantities DNA loaded the more MSNs were internalized by cancer cells. And compare with the uptake of the HeLa cells and HepG2 cells, the overall trend were similar except a little difference about the N-MSNs and the N-MSNs loaded low concentration DNA. In addition to this the cytotoxicity of the MSNs examined by MTT assay demonstrated that the MSNs possess good biocompatibility. So the MSNs can load large quantities small molecule nucleic acid and internalized by cancer cells efficiently. The findings provide a support for clinical research of gene therapy. | |||
| TO cite this article:SUN Yan,LIN Li,LIU Xianbin. The Cell Uptake of Mesoporous Silica Nanoparticles Carrying DNA in Vitro[OL].[25 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4523386 | |||
| 7. Association Between Genetic Variants in pre-miRNA and Colorectal Cancer Risk in a Chinese Population | |||
| Lv Meili,Dong Wei,Wei Yonggang,Li Lijuan,Zhang Lushun,Shu Xiaowei,Wang Li,Gao Linbo*,Zhang Lin* | |||
Basic Medicine 28 January 2013
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| Abstract:Background: Single nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA expression levels or processing and then contribute to the susceptibility of cancer development. We hypothesized that SNPs in pre-miRNAs may be association associated with the risk of colorectal cancer (CRC). Methods: We used genotyped four common polymorphisms (i.e., rs11614913, rs3746444, rs2910164, and rs2292832) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between the SNPs and the risk of CRC using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyped four common polymorphisms(rs11614913, rs3746444, rs2292832 and rs2910164) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between them and the risk of CRC assay..Results: The rs11614913 CT, TT genotypes and T allele were associated with an increased risk of CRC compared with the CC genotype and C allele (CT vs. CC: OR＝7.34, 95% CI, 3.76-14.34; TT vs. CC: OR＝13.66, 95% CI, 6.76-27.6; T vs. C: OR＝1.99, 95% CI, 1.63-2.42, respectively). Interestingly, using the rs2910164 GG genotype as a reference, the rs2910164 GC genotype was associated with an increased risk of CRC (OR＝1.49, 95% CI, 1.02-2.18), whereas the rs2910164 CC genotype was associated with a decreased risk of CRC (OR＝0.58, 95% CI, 0.37-0.93). When compared with the rs2910164G allele, rs2910164 C allele was associated with a reduced risk of CRC (OR＝0.80, 95% CI, 0.66-0.97, P=0.02). significantly increased CRC risk were found to be associated with CT genotype (OR＝0.136, 95% CI: 0.070-0.266) and TT genotype (OR＝0.073, 95% CI: 0.036-0.148) vs. CC genotype of rs11614913, CT (OR＝0.166, 95% CI: 0.037-0.735,) and TT genotype (OR＝0.167, 95% CI: 0.038-0.729) vs. CC genotype of rs3746444, and GC genotype (OR＝0.670, 95% CI: 0.460-0.977) vs. GG genotype of rs2910164. Unfortunately, there were no statistically significant differences between cases and controls in genotype of rs2292832. When compared with the alleles, significantly increased CRC risk were found to be associated with T allele (OR＝0. 530, 95% CI: 0.413-0.613) vs. C allele in rs11614913. There were no statistically significant differences between cases and controls in alleles of rs3746444, rs2292832 and rs2910164.Conculsion: These findings suggest that rs11614913 and rs2910164 polymorphisms may be associated with the etiology of CRC. | |||
| TO cite this article:Lv Meili,Dong Wei,Wei Yonggang, et al. Association Between Genetic Variants in pre-miRNA and Colorectal Cancer Risk in a Chinese Population[OL].[28 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4517990 | |||
| 8. Acetyl-11-keto-β-boswellic acid (AKBA) inhibits human gastric carcinoma growth through modulation of the Wnt/β-catenin signaling pathway | |||
| Chu Jiahui,Song Zhiyu,Lu Yuyin,Yuan Yi,Qu Xianjun | |||
| Basic Medicine 26 January 2013 | |||
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| Abstract:Background: Acetyl-11-keto-beta-boswellicacid (AKBA) is a derivative of boswellic acid, which is an active component of gum resin of Boswellia serrata. AKBA has been used as drug to treatment of inflammatory diseases like inflammatory bowel disease, rheumatoid arthritis, osteoarthritis etc. In this study, we aimed to examine the effect of AKBA on human gastric carcinoma growth, and to explore the underlying molecular mechanisms. Methods: Inhibition of cancer growth was estimated by colorimetric and clonogenic assays. Cell cycle was analyzed by FACScan flow cytometer. The apoptotic cells were determined by Annexin V-FITC/PI staining and DNA ladder quantification. Further experiment was performed in mice bearing human cancer xenografts. After 3 weeks oral administration, mice were sacrificed and the xenografts were removed for TUNEL staining and Western blotting analysis. Results: AKBA significantly inhibited cancer growth as determined in vitro and in mice. Oral AKBA in mice effectively delayed the growth of SGC-7901 xenografts without toxicity. The inhibition of AKBA on cancer growth was associated with its activity in cell cycle arrest and apoptosis induction, showing the activation of p21Waf1/Cip1 and p53 in mitochondria. More evidences included the increase of cleaved caspase-9, caspase-3 and cleaved PARP, and also the Bax/Bcl-2 ratio in AKBA-treated cells. Further analysis suggested that AKBA could impair the β-catenin-dependent expression of signals, showing decrease of β-catenin in nuclei and increase of β-catenin in membrane. Consequently, GSK3β was activated and the expressions of cyclin D1, PCNA, survivin, c-Myc, MMP-2 and MMP-7 were inhibited. Conclusions: AKBA inhibited human gastric carcinoma growth, possibly due to modulation of the Wnt/β-catenin signaling pathway. AKBA could be a useful agent in treatment of gastric cancers. | |||
| TO cite this article:Chu Jiahui,Song Zhiyu,Lu Yuyin, et al. Acetyl-11-keto-β-boswellic acid (AKBA) inhibits human gastric carcinoma growth through modulation of the Wnt/β-catenin signaling pathway[OL].[26 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4515454 | |||
| 9. Inhibition of gastric motility by endogenous H2S via enteric nervous system | |||
| YE Yanfang,LU Wen,ZHU Jianchun,DU Yahui,LIU Kejing,QIN Junfang,FENG Mei,LUO Yan,LIU Chuanyong | |||
| Basic Medicine 17 January 2013 | |||
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| Abstract:Hydrogen Sulfide (H2S) might be a gastrotransmitter in enteric nervous system and might be involved in the regulation of gastrointestinal motility and secretion. This study was conducted to investigate the effect of endogenous H2S on gastric motility. Methods. In in vivo experiments, the intragastric pressure of rats were monitored, and in in vitro experiments, the spontaneous contraction of muscle strips of gastric body was recorded. The location of cystathionine-beta-synthetase (CSE) and cystathionine-gama-lyase (CBS) in stomach was studied by immnohistochemistry staining and Western blot. Key Results: Systemic L-cysteine (100 μmol /kg，i.p.) significantly decreased intragastric pressure. In vitro, L-cysteine (10-4 M- 5×10-3 M) and S-adenosyl-l-methionine (SAM) (5 × 10-4 M - 5 × 10-3 M) significantly decreased the tension of the muscle strips from gastric body. This effect of L-cysteine on muscle strip was not influenced by pretreatment of L-NAME and glibenclamide but was reversed by TTX. In in vitro experiments, administration of PAG (10-6 M-10-3 M), the specific inhibitor of CSE, or AOAA (10-6 M), the specific inhibitor of CBS, significantly increased the mean tension of gastric muscle strips. Both PAG and AOAA significantly reversed the inhibitory effect of L-cysteine on gastric motility in vitro. Both CBS and CSE were located in the myenteric plexus of stomach. Conclusions & Inferences : We concluded that endogenous H2S inhibited gastric motility via ENS. H2S might be a physiologic relaxant of gastric smooth muscle and exert tonic inhibition on gastric motility. | |||
| TO cite this article:YE Yanfang,LU Wen,ZHU Jianchun, et al. Inhibition of gastric motility by endogenous H2S via enteric nervous system[OL].[17 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4515344 | |||
| 10. Comparison of direct boiling with commercial kits for extracting fecal microbiome DNA with Illumina sequencing of 16S rRNA tags | |||
| PENG Xin,ZHOU Hongwei,DENG Guanhua,JIANG Yunxia,WANG Yu,ZHANG Guoxia | |||
| Basic Medicine 16 January 2013
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| Abstract:High cost-efficiency and throughput are major advantages for determining metagenomic 16S rRNA tag sequences using next generation sequencing (NGS) techniques. These methods have significantly changed our view of microorganisms in fields of human health and environmental science. However, DNA extraction using commercial kits has become the major bottleneck because of its high cost and time consuming shortcomings. In the present study, we evaluated the determination of fecal microbiome using a direct boiling method compared to 5 different kinds of commercial methods, e.g. Qiagen and MO BIO kits. The PCoA analysis using UniFrac distances and the clustering result showed that direct boiling with a wide range of feces concentration had similar bacterial communities to most of the commercial kits, except the MO BIO method. The fecal concentration for boiling affected the estimation of α-diversity indices, but the results were generally comparable for boiling and commercial methods. The OTUs determined by direct boiling showed highly consistent frequencies with those determined by most of the commercial methods. Even for the MO BIO kit with an obviously different community structure, most of the OTUs determined at high to medium levels by the MO BIO kit were also determined in the direct boiling results with high confidences. Our present study suggested that direct boiling could be used to determine the fecal microbiome, and would significantly reduce the cost and improve the efficiency of the sample preparation for studying gut microbiome diversity.an) | |||
| TO cite this article:PENG Xin,ZHOU Hongwei,DENG Guanhua, et al. Comparison of direct boiling with commercial kits for extracting fecal microbiome DNA with Illumina sequencing of 16S rRNA tags[OL].[16 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4513274 | |||
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