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	1. Differentiation of embryonic stem cells into hepatocytes induced by a combination of cytokines and Trichostatin A
	Zhu Xiaobo,ZHANG Dongmei,ZHANG Qian,ZHANG Jie,ZHOU Mingming
	Biology 30 October 2013
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	Abstract:In this paper，we presented a novel 3-step procedure to efficiently direct the differentiation of mouse ESCs into hepatocytes induced by combination of cytokines and sodium butyrate. Mouse ESCs were first differentiated into definitive endoderm cells by 3 days of Activin A treatment; next, presence of acid fibroblast growth factor(aFGF)and Trichostatin A (TSA)in the culture medium for 5 days, definitive endoderm cells efficiently differentiated to hepatocytes. After 10 days of further in vitro maturation, the morphological and phenotypic markers of hepatocytes were characterized using light microscopy, immunofluorescence and RT-PCR, respectively. Furthermore, these cells were tested about the functions associated with mature hepatocytes including glycogen storage, indocyanine green uptake and release,and the ratio of hepatic differentiation was determined by counting the albumin-positive cells，which showed that the ratio of hepatic differentiation was 57.38%． The ES cell differentiating method provides a new resource for hepatocytes tansplatation．
	TO cite this article:Zhu Xiaobo,ZHANG Dongmei,ZHANG Qian, et al. Differentiation of embryonic stem cells into hepatocytes induced by a combination of cytokines and Trichostatin A[OL].[30 October 2013] http://en.paper.edu.cn/en_releasepaper/content/4566643


	2. Transcriptome analysis of murine thymic epithelial cells reveals age-associated changes in microRNA expression
	Guo Zhibin,Chi Feng,Song Yan,Wei Tianli,Zhu Xike
	Biology 20 August 2013
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	Abstract:Age-related thymic involution is accompanied by a decrease in thymopoiesis and thus deficiency in T-cell-mediated immunity in the elderly. Many events involved in thymic involution have been discovered; however, it is unclear if they are causes or consequences of thymic involution. These events include the degeneration of T-cell progenitors as well as the deterioration of the thymic stroma, which is a characteristic of thymic epithelial cell loss due to increased apoptosis and decreased cell proliferation. MicroRNA (miRNA) is believed to play important roles in regulating cell death and proliferation in the aging network. The aim of this study is to profile the miRNA expression in thymic epithelial cells and delineate the change in the expression level along with thymic aging. By comparing the expression levels of various miRNAs in thymic epithelial cells and their changes in different age groups, this study provides first-hand information about the changes of miRNA expression in the thymic stroma with aging.
	TO cite this article:Guo Zhibin,Chi Feng,Song Yan, et al. Transcriptome analysis of murine thymic epithelial cells reveals age-associated changes in microRNA expression[OL].[20 August 2013] http://en.paper.edu.cn/en_releasepaper/content/4556213

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	3. The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2
	FEI Fei,ZHU Daoli,TAO Lijun,HUANG Baozhu,ZHANG Honghong
	Biology 21 April 2013
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	Abstract:Objective To investigate the protective effect of ATP on skeletal muscle satellite cells of newborn rats damaged by H2O2 and its influence on the related proteins Bax and Bcl-2. Methods The H2O2-induced damage models of skeletal muscle satellite cells of newborn rats were established and the study was divided into 4 groups：normal control group，model control group (0.1 mmol / L H2O2 solution damaged cells for 50s)，protection group(10，5，2.5，1.25，0.625，0.3125，0.15625 mg/mL ATP solutions treated cells and then 0.1 mmol / L H2O2 solution damaged cells for 50s)，proliferation group (10，5，2.5，1.25，0.625，0.3125，0.15625 mg/mL ATP solutions treated cells). MTT colorimetric analysis，FITC+PI+DAPI fluorescent staining，Giemsa staining and immunocytochemistry fluorescent apoptotic antibody test were applied to detect the survival rate of cells and the protective effect of ATP on skeletal muscle satellite cells damaged by H2O2. Results The survival rate of skeletal muscle satellite cells was decreased and the apoptosis rate was increased after damaged. The survival rate of muscle satellite cells protected by the ATP solution could be increased (P<0.01), and the apoptosis rate was decreased. Immunocytochemistry staining analysis results showed that ATP solution could enhance Bcl-2 proteins (P<0.01) and inhibite Bax proteins (P<0.01). Different doses of ATP solutions had different effects on skeletal muscle satellite cells induced by H2O2: the survival rate of muscle satellite cells protected by the adequate doses of ATP solutions （2.5、1.25、0.625 mg/mL）could be increased. The protective effect on cells treated by 1.25 mg/mL ATP solution was the most obvious. Cells treated by 10，5mg/mL ATP solutions could even cause obvious apoptosis. Conclusion The ATP solution had obvious protective effect on skeletal muscle satellite cells damaged by H2O2 in new-born rats. The main mechanism of ATP solution was related to strengthen the expression of Bcl-2 and inhibit that of Bax through JAK -STAT signal transduction, thus inhibiting apoptosis of cells.
	TO cite this article:FEI Fei,ZHU Daoli,TAO Lijun, et al. The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2[OL].[21 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4539356


	4. Effects of dibutyl phthalate on ovarian active substance of mice
	WANG Yu,WANG Shengqing,YE Wengbin,HE Jiujun
	Biology 15 March 2013
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	Abstract:With superoxide dismutase(SOD), catalase(CAT), alkaline phosphatase(AKP), acid phosphatase(ACP), malondialdehyde(MDA) and Bax protein expression as characteristic indexes, the effects of dibutyl phthalate(DBP) on ovarian active substance of mice were studied, and the toxic mechanisms were discussed. One hundred sixty mice were given intragastric administration of DBP (0, 100, 250, or 500 mg/(kgod)), respectively, for 30 days. At 5, 10, 20, 30 days after DBP exposure, the ovarian examinations were taken. The results revealed that administration of DBP decreased the activities of SOD, CAT, AKP and ACP, while MDA content and Bax protein expression increased. Two-way analysis of variance indicated that dose contributed more to DBP-induced ovarian damage than treatment time. The present study demonstrated that DBP promoted apoptosis and inhibited antioxidant capacity of the ovarian.
	TO cite this article:WANG Yu,WANG Shengqing,YE Wengbin, et al. Effects of dibutyl phthalate on ovarian active substance of mice[OL].[15 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4527462


	5. The noncoding RNA Llme23 drives the malignant property of human melanoma cells
	Wu Chuanfang,tanguanghong,machengchuan,liling
	Biology 26 February 2013
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	Abstract:Several lines of evidence support the notion that increased RNA-binding ability of Polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF) and aberrant expression of long noncoding RNAs (lncRNAs) are associated with mouse and human tumors. To identify the PSF-binding lncRNA involved in human oncogenesis, we screened a nuclear RNA repertoire of human melanoma line, YUSAC, through RNA-SELEX affinity chromatography. A previously unreported lncRNA, termed as Llme23, was found to bind immobilized PSF resin. The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines. Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells, accompanied by the repressed expression of proto-oncogene Rab23. These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding lncRNA to human melanoma.
	TO cite this article:Wu Chuanfang,tanguanghong,machengchuan, et al. The noncoding RNA Llme23 drives the malignant property of human melanoma cells[OL].[26 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4522449


	6. Cloning and purifying of Soluble amyloid precursor protein and its modulating MAPK signal pathway
	CHEN Keping,DOU Fei
	Biology 04 January 2013
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	Abstract:It is well known that the proteolytic processing of the amyloid precursor protein (APP) release β-amyloid (Aβ), which plays a central role in the pathogenesis of Alzheimer's disease (AD). Understanding APP processing is important for reducing Aβ levels in AD therapeutic strategy. Soluble amyloid precursor protein (sAPPa) is a proteolyte of APP cleavage by α-secretase. The significance of the cleavage and the physiological functions of sAPPα are poorly understood. In this study, we constructed the stable cell lines expressing sAPPα fusion protein and purified with tandem affinity purification (TAP) technology. We also found sAPPα could modulate the MAPK signal pathway involving in NCAM-induced neurite outgrowth, likes the full length APP.
	TO cite this article:CHEN Keping,DOU Fei. Cloning and purifying of Soluble amyloid precursor protein and its modulating MAPK signal pathway[OL].[ 4 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4509550


	7. Aberrant Expression of ZNF268 Alters the Growth and Migration of Ovarian Cancer Cells
	HU Li,WANG Wei,CAI Jinyang,LUO Jun,HUANG Yi,XIONG Shilu,LI Wenxin,GUO Mingxiong
	Biology 29 December 2012
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	Abstract:Ovarian cancer is one of the most lethal gynaecologic cancers worldwide. However, the mechanism underlying ovarian carcinogenesis is not well understood. Here, we show that ZNF268 is overexpressed in human ovarian carcinomas. ZNF268 knockdown increased the viability, colony formation, and the growth of in vivo xenografts of ovarian carcinoma SKOV-3 cells, whereas SKOV-3 cell migration was inhibited by ZNF268 knockdown. Furthermore, we demonstrated that ZNF268 knockdown may increase SKOV-3 cell growth by promoting cell cycle progression. Our findings suggest that ZNF268 is a novel protein involved in ovarian carcinogenesis and may help us better understand the mechanism of ovarian carcinogenesis.
	TO cite this article:HU Li,WANG Wei,CAI Jinyang, et al. Aberrant Expression of ZNF268 Alters the Growth and Migration of Ovarian Cancer Cells[OL].[29 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4509597


	8. SphK1/S1P/S1PR1 signaling stimulates cell migration in RAW264.7 macrophages
	Li Changyong
	Biology 27 August 2012
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	Abstract:Macrophage recruitment to sites of inflammation is an essential step in host defense. However, the signals regulating the mobilization of these cells are still not fully understood. Sphingosine-1-phosphate (S1P), a pleiotropic bioactive lipid mediator, is known to regulate an array of biological activities in various cell types. Here, we investigated the roles of S1P and S1P receptors (S1PRs) in macrophage migration in vitro. Furthermore, we explored the cross-talk between transforming growth factor-β1 (TGF-β1) and S1P signaling pathways in this process. We found that S1P exerted a powerful migratory action on RAW264.7 macrophages, as determined in Boyden chambers. Moreover, by employing RNA interference technology and pharmacological tools, we have demonstrated that S1PR1, but not S1PR2 and S1PR3, is required for S1P-induced macrophage migration. Importantly, we observed a pronounced increase in sphingosine kinase 1 (SphK1) mRNA expression and subsequently increase in S1P production, following transforming growth factor-β1 (TGF-β1) stimulation in RAW264.7 macrophages. The expression of S1PR1, but not S1PR2 and S1PR3, was also significantly up-regulated after TGF-β1 stimulation. Interestingly, exogenously added S1P induced up-regulation of SphK1 and the synthesis of additional S1P, suggesting a self-amplifying loop of S1P to enhance macrophage migration. In conclusion, our results reveal that SphK1/S1PR1 signalling axis is induced by TGF-β1 and stimulates cell migration in RAW 264.7 macrophages. This study provides new clues for the molecular mechanisms of macrophage recruitment during inflammation.
	TO cite this article:Li Changyong . SphK1/S1P/S1PR1 signaling stimulates cell migration in RAW264.7 macrophages[OL].[27 August 2012] http://en.paper.edu.cn/en_releasepaper/content/4487743


	9. Effects of truncated mutation of APC on cell-matrix and cell-cell adhesion in kidney epithelial cell lines
	LI Wenling,ZHU Wensi,NIU Haibo,SONG Li,LI Zhuoyu
	Biology 18 April 2012
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	Abstract: APC is associated with cell adhesion, but the effects of truncted APC and the mechanism are not well defined.To explore the impact of mutant adenomatous polyposis coli (APC) on cell-matrix, cell-cell adhesion and the relative mechanism. Cell-matrix and cell-cell adhesion assay were employed to determine the adhesion level of two stable cell lines MDCK-N2-APC and MDCK-GFP. The truncated APC of N2 fragment, which spans residues 449-781 was studies compared to control cells including GFP alone. The immunofluorescence staining, RT-PCR analysis and Western blotting were applied to check several adhesion molecular which have key roles in cell contacts process. In contract with control, cell-matrix adhesion was averagely increased to180% in N2 cells, whereas, cell-cell adhesion was reduced by about 30%. Our experiments indicated that N2 fragment of APC decreases cell adhesion via enervating E-cadherin expression level. It enhances cell adhesion by means of improving CD29 level respectively. These data suggest that full length APC plays a crucial role in cell-matrix adhesion and cell-cell adhesion. The truncation mutation of APC fragment, N2 restrained in the colon cancer cells, will alter the cell invasion and migration by the influence on cell adhesion and cell- matrix adhesion
	TO cite this article:LI Wenling,ZHU Wensi,NIU Haibo, et al. Effects of truncated mutation of APC on cell-matrix and cell-cell adhesion in kidney epithelial cell lines[OL].[18 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4475722


	10. ESThfl22w: ESTs database on human fetal liver aged 22 wk of gestation
	CHEN Tinggui,ZAN Yawei,LU Haigang
	Biology 18 August 2011
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	Abstract:Human fetal liver aged 22 weeks of gestation (HFL22w) consists of hepatic parenchymal cells and hematopoietic stem/progenitor cells, not only has liver functions, but also has hematopoietic functions, and is a turning point between immigration and emigration of hematopoietic system. In this article, the EST data were sequenced, and clustered by blasting against five human transcriptome databases. Then after EST assembly and gene identification, the known genes were classified by GO, and the unknown genes were analyzed by Pfam and ScanProsite to predicted their functions. This database is comprised of six parts: introduction, methods, significance, known genes, unknown genes and download. The results show that: (1) 12368 high-quality ESTs were obtained in 20282 sequences. (2) In 12368 ESTs, 8097 ESTs were matched to 2483 known genes, and 4271 ESTs were identified as 2416 genes that exhibited no significant homology to known genes. (3) In 2416 genes, 1379 genes were identified as fully new sequences. (4) Through GO classification, 6 cell migration genes and 6 hemopoiesis genes were confirmed. (5) Prediction of gene function had enabled us to obtain 277 profiles. Among them, there are five categories distributed in more than 10 genes. (6) We have built the world's biggest EST database on HFL22w. This database on HFL22w will help to understand hemopoiesis and cell migration mechanism, and promote future study on HFL22w for all interested researcher. ESThfl22w can be freely accessed at http://www.ikaixue.com/chen.
	TO cite this article:CHEN Tinggui,ZAN Yawei,LU Haigang. ESThfl22w: ESTs database on human fetal liver aged 22 wk of gestation[OL].[18 August 2011] http://en.paper.edu.cn/en_releasepaper/content/4438819

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