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	1. Characterization of different 2b of Cucumber mosaic virus based on silencing suppressor
	Li Xiying ,Zhao Zhijing,Du Zhiyou,Chen Jishuang
	Biology 08 February 2010
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	Abstract:FRad35Leu2b-CMV is a spontaneous mutant from an attenuated strain FRad352b-CMV, which differs in a single amino acid from the 2b of FRad352b-CMV. FRad35Leu2b-CMV induced high pathogenicity and high viral progeny RNAs accumulation, even can revert the PTGS of GFP in the newly-developed leaves. We further show that the FRad35Leu2b-CMV inhibits the Nicotiana benthamiana AGO1-1, AGO4-1 transcription rather than FRad352b-CMV. Then we demonstrate that the PTGS suppressor (2bLeu) of FRad35Leu2b-CMV could not only suppress local gene silencing, but also suppress systemic gene silencing through blocking systemic movement of viral siRNA. Here we consider the 2bLeu interferes with the PTGS pathway by suppressing the movement of siRNAs. Taken together, we speculate that high virulence mediated by 2bLeu is achieved through blocking systemic movement of viral siRNA, and inhibiting RdDM by down-regulating AGO4-1, AGO1-1 expression.
	TO cite this article:Li Xiying ,Zhao Zhijing,Du Zhiyou, et al. Characterization of different 2b of Cucumber mosaic virus based on silencing suppressor[OL].[ 8 February 2010] http://en.paper.edu.cn/en_releasepaper/content/40057


	2. Characterization of different 2b of Cucumber mosaic virus based on silencing suppressor
	Li Xiying,Zhao Zhijing,Du Zhiyou,Chen Xiang,Chen Jishuang
	Biology 22 December 2009
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	Abstract:Rad35Leu2b-CMV is a spontaneous mutant from an attenuated strain FRad352b-CMV, which differs in a single amino acid from the 2b of FRad352b-CMV. FRad35Leu2b-CMV induced high pathogenicity and high viral progeny RNAs accumulation, even can revert the PTGS of GFP in the newly-developed leaves. We further show that the FRad35Leu2b-CMV inhibits the Nicotiana benthamiana AGO1-1, AGO4-1 transcription rather than FRad352b-CMV. Then we demonstrate that the PTGS suppressor (2bLeu) of FRad35Leu2b-CMV could not only suppress local gene silencing, but also suppress systemic gene silencing through blocking systemic movement of viral siRNA. Here we consider the 2bLeu interferes with the PTGS pathway by suppressing the movement of siRNAs. Taken together, we speculate that high virulence mediated by 2bLeu is achieved through blocking systemic movement of viral siRNA, and inhibiting RdDM by down-regulating AGO4-1, AGO1-1 expression.
	TO cite this article:Li Xiying,Zhao Zhijing,Du Zhiyou, et al. Characterization of different 2b of Cucumber mosaic virus based on silencing suppressor[OL].[22 December 2009] http://en.paper.edu.cn/en_releasepaper/content/37897

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	3. A Novel Tetracycline-Suppressed Expression System Based on Single Lentiviral Vector
	Yang Chunyan ,Liu Jia,Wang Chunhong,Sun Yan,Xu Zenghui,Zhou Chengliang,Lei Qiugang,Wu Mengchao,Qian Qijun
	Biology 01 December 2009
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	Abstract:Tetracycline-regulated eukaryotic expression system is a useful tool in area of biological researches. However, the current system is characterized of separation of essential elements into two individual vectors, resulting in inconvenience in the process of application. Here, we successfully generated single-vector tetracycline-regulated expression system which is based on recombinant lentivirus. Our data confirmed the efficiency and reliability of this single-vector controllable expression system. We hope that our work would advance the application of tetracycline-regulated system in future.
	TO cite this article:Yang Chunyan ,Liu Jia,Wang Chunhong, et al. A Novel Tetracycline-Suppressed Expression System Based on Single Lentiviral Vector[OL].[ 1 December 2009] http://en.paper.edu.cn/en_releasepaper/content/37123


	4. Expression Analysis and Characteristics of Prohibitin Gene from Silkworm, Bombyx mori
	Zhang Xuefang,Zhuang Wenhua,Lv Zhengbing,Zhang Yaozhou
	Biology 24 November 2009
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	Abstract:Prohibitin (PHB) is an evolutionarily conserved ubiquitously expressed multifunctional protein. However, its mechanism of action is unknown as yet. To better characterize the prohibitin protein from silkworm pupae (BmPHB), a gene encoding a prohibitin protein was identified from a cDNA library of silkworm pupae, which has an ORF of 825 bp, encoding a predicted 274 amino acids. A His-tagged BmPHB fusion protein was expressed in Escherichia coli Rosetta (DE3) and purified with affinity and reversed-phase chromatographies. Purified rBmPHB was used to generate anti-BmPHB polyclonal antibody, which was used to determine the subcellular localization of BmPHB. Immunostaining indicated that prohibitin can be found in both nucleus and cytoplasm but is located primarily in cytoplasm. Western blot analyses indicated that, in the fifth instar larva, BmPHB was expressed descendingly in gonad, malpighian tubule, trachea, fatty body, intestine and head. However, none was detected in larva’s silk gland and epidermis. Additionally, BmPHB was expressed in the nascent egg, larva and pupa, but none was detected in the moth. Therefore, we hypothesized that BmPHB may play an important role on silkworm development. This is a first description of the prohibitin protein from silkworm pupae, B. mori.
	TO cite this article:Zhang Xuefang,Zhuang Wenhua,Lv Zhengbing, et al. Expression Analysis and Characteristics of Prohibitin Gene from Silkworm, Bombyx mori[OL].[24 November 2009] http://en.paper.edu.cn/en_releasepaper/content/36970


	5. Expression, Purification and Characterization of extramacrochaetae (emc) from the silkworm
	Yang Qing ,Zeng Fenghui,Lv Zhengbing,Zhang Yaozhou
	Biology 16 November 2009
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	Abstract:The Drosophila extramacrochaetae protein is one of the Helix-loop-Helix superfamily proteins, which have an effect on the expression of many genes during Drosophila development. We expressed the Bombyx mori extramacrochaetae gene (Bmemc) in E. coli BL21. After purification with affinity chromatography, the fusion protein was used to prepare the polyclonal antibody. We have detected the expression of Bmemc in mRNA and protein levels. The results showed that the expression levels was highest in the tissues of testis, followed by the ovary, head, midgut and trachea; the protein was barely detectable in the Malpighian tuble, silk gland and fat body. Bmemc is highly expressed in moth, fifth instar larvae and pupae, but lower in egg. Based on the result of Notch signaling assay, we proposed that Bmemc gene may be regulated by Notch signaling pathway.
	TO cite this article:Yang Qing ,Zeng Fenghui,Lv Zhengbing, et al. Expression, Purification and Characterization of extramacrochaetae (emc) from the silkworm[OL].[16 November 2009] http://en.paper.edu.cn/en_releasepaper/content/36741


	6. Evolutionary characterization of two Cucumber mosaic virus isolates infecting Pinellia ternata of Araceae family
	HaiYan Wang,HuaRong Zhang,ZhiYou Du,Rong Zeng,JiShuang Chen
	Biology 26 February 2009
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	Abstract:Two Cucumber mosaic virus (CMV) isolates，PHz-CMV and PGs-CMV, were obtained from naturally infected Pinellia ternata as a Chinese medicinal plant belonging to Araceae family. Phylogenetic analysis showed that the pinellia isolates formed an independent clade with high support within subgroup I. Pairwise comparison analysis showed that they had high divergence from subgroup strains of IA and IB, Remarkable Difference revealed their relationships with subgroup IA and IB. Along with their RNA3 5′UTRs analysis, it is demonstrated that they may have independent evolution. It is believed that host and geographical environment had impact on evolutional types of this virus. A novel CMV subgroup is suggested based on the discussion.
	TO cite this article:HaiYan Wang,HuaRong Zhang,ZhiYou Du, et al. Evolutionary characterization of two Cucumber mosaic virus isolates infecting Pinellia ternata of Araceae family[OL].[26 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29724


	7. Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus
	Qinghua Tian,Liqing Li,Jishuang Chen
	Biology 26 February 2009
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	Abstract:Two double-stranded RNAs were extracted from leaf tissues of Raphanus sativus-root cv. Yidianhong grown in Eastern China. Their full sequences, namely RasR7 and RasR8, were determined by a single primer amplification technique. Comparisons of 5′ untranslated regions (UTR) and 3′ UTR between RasR7 and RasR8 showed that they were highly conserved at 5′ UTR and an identical region 5′-AGAAUUU-3′ was discovered as the evidence of the same plant virus. 5′-UAAGAC-3′ was found as their identical region in 3′ UTR. BLASTP search in Genbank showed that RasR7 encoded putative protein was much similar with RNA dependent RNA polymerases (RdRps) of plant-infecting dsRNA viruses belonging to the family Partitiviridae. Phylogenetic analysis showed that RasR7 encoded putative protein clustered with the RdRps of members of the family Partitiviridae. RT-PCR detection indicated that both RasR7 and RasR8 existed in the virus-like particles. Therefore, it was suggested that RasR7 and RasR8 institute the genome of a newly discovered cryptic virus, namely Raphanus sativus cryptic virus 3 (RasV3).
	TO cite this article:Qinghua Tian,Liqing Li,Jishuang Chen. Complete nucleotide sequences of two double-stranded RNAs from Raphanus sativus-root demonstrate the incidence of a novel cryptic virus [OL].[26 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29713


	8. Transcriptional Profiling of Class A Rice Heat Stress Transcription Factors
	Ailing Liu,Xinbo Chen,Jie Zou,Xianwen Zhang,Xiaoyun Zhou,Wenfang Wang,Liyun Chen,Xinyao Xiong
	Biology 10 February 2009
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	Abstract:Expression profiles of twelve class A rice heat shock transcription factors genes (OsHsfA) were analyzed by semi-quantitative RT-PCR. The OsHsfA genes exhibited distinctive expressions in different organs and were affected differentially under abiotic stresses. Except for OsHsfA1a and OsHsfA3, all other OsHsfA family genes were strongly up-regulated under heat treatments; the majority of OsHsfA expression responses to salt, osmotic, and cold treatment primarily occur at the late stages of stress exposure. Our investigation implied that the twelve OsHSF genes may play different roles in plant development and abiotic stress responses.
	TO cite this article:Ailing Liu,Xinbo Chen,Jie Zou, et al. Transcriptional Profiling of Class A Rice Heat Stress Transcription Factors [J].


	9. Expression Profile of Young Rice Panicle under Heat Stress using DNA Microarray
	Xianwen Zhang,Ailing Liu,Jie Zou,Xiaoyun Zhou,Wenfang Wang,Liyun Chen,Xingyao Xiong,Xinbo Chen
	Biology 16 January 2009
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	Abstract:Heat shock has become one of the most serious stress factors to world crop yield and quality, and many genes responsive to heat stress and other abiotic and biotic stresses have been discovered and their functions have been elucidated. However, plant genome wide heat response mechanism still remains unclear. Here, we reported expression profile of young rice panicle under heat stress of 40°C on the whole genome level using rice 44k oligo-array. The results demonstrated that carbohydrate metabolism and stress-related genes consist of major group that are affected during the time course. Hsps, Hsfs, enzymes responsible for carbon partitioning, secondary metabolism, cell wall morphogenesis- related genes, and ETC are all sensitive to heat, and then the stress shed influence on DNA replication, and next on cell homeostasis, reproduction, and finally on cell cycle and photosynthesis. We tried to establish heat response model for rice panicle, which would be valuable to crop production and improvement.
	TO cite this article:Xianwen Zhang,Ailing Liu,Jie Zou, et al. Expression Profile of Young Rice Panicle under Heat Stress using DNA Microarray[J].


	10. Expression of five trypsins from the Chinese shrimp Fenneropenaeus chinensis as regulated by pathogenic infection
	Shi XiuZhen,Ren Qian,Zhao XiaoFan,Wang JinXing
	Biology 14 January 2009
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	Abstract:Five trypsins designated as Fctry1-1, Fctry1-2, Fctry2，Fctry3, and Fctry4 were obtained from the hepatopancreas of the Chinese shrimp Fenneropenaeus chinensis. Accordingly, four Fctrypsins, namely, Fctry1-1, 1-2, 2, and 3, were observed to have conserved the catalytic triad (H, D, and S), while in Fctry4, one of the catalytic triad S was substituted by F, and the mature peptide was found to be inactive. Further analysis for similarities was also conducted, with the results indicating that the characteristics of these trypsins are identical with those of trypsins from the Pacific white shrimp (Litopenaeus vannamei), other shrimp, and krill. Moreover, semi-quantitative reverse transcriptional polymerase chain reaction (PCR) was carried out to analyze the expression pattern and distribution profiles of the trypsins after they were subjected for bacterial and viral challenge. The results showed that in the transcriptional level, these trypsins were upregulated in hepatopancreas after they were infected with bacteria and virus. Therefore, these trypsins might be involved in the innate defense reactions manifested by Chinese shrimp.
	TO cite this article:Shi XiuZhen,Ren Qian,Zhao XiaoFan, et al. Expression of five trypsins from the Chinese shrimp Fenneropenaeus chinensis as regulated by pathogenic infection[OL].[14 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27816

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