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1. Effect of solanine on cell apoptosis by regulating Bcl-2/Bax ratio in HepG2 cell | |||
JI Yubin,GAO Shi-yong | |||
Traditional Chinese Medicine and Pharmacology 10 January 2012 | |||
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Abstract:Objective To explore the anti-tumor effect of solanine and the mechanisms involved. Methods The in vivo anti-tumor effect of solanine is observed using models developed through in vivo transplantation of tumor cells; in vitro lines of sensitive anti-tumor cells are selected from the digestive system using MTT assay; the effect of solanine on cell morphology is observed using transmission electronic microscopy; the morphology of apoptotic cells is observed using Annexin V/PI double staining and laser confocal scanning microscopy; the rate of cell apoptosis is measured using Annexin V/PI double staining and flow cytometry; the concentration of intracellular Ca2+ is determined using Fluo-3/AM staining and laser confocal scanning microscopy; the membrane potential of cellular mitochondria is determined using TMRE staining and laser confocal scanning microscopy; the protein expression of Bcl-2 and Bax is measured using immunological marking and laser confocal scanning microscopy; and the activity of caspase-3 is measured using the colorimetric method. Results Solanine can inhibit the growth of tumor weight in S180 tumor-bearing mice and prolong the survival time of H22 tumor-bearing mice. Selection by MTT assay reveals that HepG2 cells are quite sensitive to solanine, for solanine can induce morphological changes characteristic of apoptotic cells in HepG2 cells, with the rate of early apoptosis being 4%, 8.5%, and 20.1%, for HepG2 cells treated for 24 h with solanine of concentrations of 0.4, 2, and 10μg, respectively. Solanine can raise the concentration of intracellular Ca2+ and lower the membrane potential. It can reduce the protein expression of Bcl-2 while increasing that of Bax, thus increasing the activity of caspase-3. Conclusion Solanine demonstrates clear anti-tumor activity, inhibiting human hepatocarcinoma. This inhibitory effect is achieved through solanine’s decreasing the Bcl-2/Bax ratio, thus increasing the concentration of intracellular Ca2+, which turns on the enzymatic activity of the caspase family, thus inducing the apoptosis of HepG2 cells. | |||
TO cite this article:JI Yubin,GAO Shi-yong. Effect of solanine on cell apoptosis by regulating Bcl-2/Bax ratio in HepG2 cell[OL].[10 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4458765 |
2. Traditional Chinese Surgical Treatment “suture dragging combined with pad compression” for anal fistulae with secondary tracks and abscess | |||
Lu Jingen,Wang Chen | |||
Traditional Chinese Medicine and Pharmacology 31 December 2011 | |||
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Abstract:Purpose This study aimed to evaluate the efficacy and safety of traditional Chinese surgical treatment "suture dragging combined with pad compression" vs fistulotomy for anal fistulae with secondary tracks and abscess. Methods From January 2008 to March 2009, 60 patients with intersphincteric or transsphincteric anal fistulas with secondary tracks and abscess were randomly divided into study group (suture dragging combined with pad compression, SDPC) and control group (fistulotomy, F). The time of healing, recurrence rate, postoperative pain (VAS), patient satisfaction,incontinence evaluation and anorectal manometry before and after treatment were all examined. Results The time of healing (SDPC 24.33d vs F 31.57d; p<0.01) was significantly shorter and patient satisfaction score (SDPC 4.07 vs F 3.37; p<0.05) was significantly higher in the SDPC group. There were no significant differences between both groups regarding age, gender, fistulae type, mean postoperative pain scores, recurrence rate, incontinence evaluation and anorectal manometry (including maximal anal squeeze pressure and anal resting pressure) before and after treatment. Conclusions Traditional Chinese surgical treatment "suture dragging combined with pad compression" for anal fistulae with secondary tracks and abscess is less invasive, safe and effective. | |||
TO cite this article:Lu Jingen,Wang Chen. Traditional Chinese Surgical Treatment “suture dragging combined with pad compression” for anal fistulae with secondary tracks and abscess[OL].[31 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4458139 |
3. CDH1 gene polymorphisms and risk of gastric cancer associated with tongue coating appearance in a chinese population | |||
ZHAN Zhen,WU Juan,ZHANG Junfeng,YANG Yaping,TONG Shujuan,ZHANG Chunbing,LI Jin,YANG Xuewen,DONG Wei | |||
Traditional Chinese Medicine and Pharmacology 15 November 2011 | |||
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Abstract:Objective: Tongue coating appearances have always thought to be related with the progress of gastric caner in Chinese medicine. The genetic polymorphisms in E-cadherin gene (CDH1) may affect invasive/metastatic development of gastric cancer by altering gene transcriptional activity of epithelial cell. The main concern of this paper was to explore the associations among CDH1 gene polymorphisms, tongue coating appearance and predisposition of gastric cancer. Methods: Four potentially functional polymorphisms (rs13689, rs1801552, rs16260 and rs17690554) of the CDH1 gene were genotyped in a case-control study of 387 incident gastric cancer cases and 392 healthy controls by polymerase chain reaction-ligation detection reaction (PCR-LDR) method and the information of tongue coating was also collected. In order to simplify the tongue diagnosis for easily understood by biomedical professionals, only two typical tongue coating color (white and yellow) and three tongue coating amount (thick, thin and none) were identified for this analysis. Results: None of the four polymorphisms or their haplotypes achieved significant difference in their distribution between gastric cancer cases and controls. Multiple logistic regression analyses revealed that gastric cancer risk was not significantly associated with the variant genotypes of the four CDH1 polymorphisms as compared with their wild-type genotypes. However, combined the analyses of tongue coating appearance, we found, interestingly, that carrying C allele of rs13689 and G allele of rs17690554 could increase the risk of gastric cancer (OR=2.576, 95%CI=1.227-5.406 for rs13689; OR=2.154, 95%CI=1.014-4.574 for rs17690554, respectively) in individuals with yellow tongue coating. Conclusions: Our results indicate, for the first time, that genetic variants at CDH1 gene may play a role in gastric cancer preposition in consideration of specific tongue appearance in China. | |||
TO cite this article:ZHAN Zhen,WU Juan,ZHANG Junfeng, et al. CDH1 gene polymorphisms and risk of gastric cancer associated with tongue coating appearance in a chinese population[OL].[15 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4450077 |
4. Carboxymethylpachymaran Enhance Immunologic Function of Dendritic Cells Cultured In Two Kinds of Hepatoma Carcinoma Cell Line's Supernatant Related to NF-κB/Rel Pathway* | |||
YU Bin,CHEN Zhuo,WU Xian-lin,DAI Cong-qi,QIAN Guo-qiang,YU Jian-zhong,HE Hai-bin,WANG Zhi-xin,HOU Jun,CHEN Xiao-yin | |||
Traditional Chinese Medicine and Pharmacology 29 October 2011 | |||
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Abstract:Objective: To study the immunologic function of dendritic cells cultured in two kinds of hepatoma cell line's supernatant, and the enhancement effects of carboxymethylpachymaran (CMP) on dendritic cells (DCs). Methods: DCs were harvest after stimulated by granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) from umbilical cord blood using density gradient centrifugation method. Cultured supernatant of two hepatoma cell lines (HepG2 and HepG2.2.15) were collected for condition medium (CM) according to a volume ratio of supernatant to un-complete RPMI-1640 which was 3:1. CMP was dissolved in un-complete RPMI-1640 medium. Experimental groups were divided according to the culture medium, eithor condition medium or with CMP in it. DCs subsets cluster of differentiation 83 (CD83), CD86, CD1a and d-related human leukocyte antigens (HLA-DR) were analyzed by flow cytometry. The proliferation ability of allogeneic T cells in mixed lymphocyte reaction stimulated by DCs was examined by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) analysis. IL-12p70, interferon γ (IFN-γ) and nuclear factor kappa B (NF-κB) were detected by enzyme-linked immunosorbent assay (ELISA) analysis. Results: The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of condition medium were lower than those of normal group (P<0.01). Compared with normal group, groups treated with CMP showed a higher expression level of DCs subsets, lymphocytes reproductive activity, as well as IL-12 and IFN-γ secretion levels. Groups treated with CMP also demonstrated higher levels of DCs phenotype expression, and IL-12 and IFN-γ secretion in supernatant of mixed lymphocyte reaction (MLR) and hgher lymphocytes reproductive activity as compared with CM group (P<0.05). Compared with the normal group, the expression level of NF-κB in DCs nuclear was higher in CMP groups, but lower in two CM groups (P<0.05). After CMP was added, the NF- κB expression levels of two CM groups were increased compared to those before adding (P<0.05). However, there was no significant difference between two CM groups (P>0.05). Conclusion: Two kinds of hepatoma cell line's supernatant can inhibit the immunologic function of DCs. And this suppressive effect may be related to the inhibition of NF-κB/Rel pathway. CMP may up-regulate the DCs function by activating the NF-κB/Rel pathway. | |||
TO cite this article:YU Bin,CHEN Zhuo,WU Xian-lin, et al. Carboxymethylpachymaran Enhance Immunologic Function of Dendritic Cells Cultured In Two Kinds of Hepatoma Carcinoma Cell Line's Supernatant Related to NF-κB/Rel Pathway*[J]. |
5. The antidepressant-like effect of Cynanchum otophyllum | |||
YANG Qiongxiong,HUANG Xiao-yan | |||
Traditional Chinese Medicine and Pharmacology 17 August 2011 | |||
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Abstract:In the course of screening for antidepressant from medicinal plants by the despair mice models, the total glycosides from Cynanchum otophyllum (TGCO) showed potent antidepressant effect by using fluoxetine as positive control. The mice were administered intragastrically with TGCO once a day for 5 days at doses of 10 mg/kg, 20mg/kg, 40mg/kg and 80mg/kg. Treating with TGCO significantly decreased the mice immobility time in tail suspension test (40, 80mg/kg, p<0.01) and in forced swim test (80mg/kg, p<0.01). Compared with blank control group, the treatment with TGCO decreased the traveled distance during test without statistical signi?cantly in the locomotor activity test. Treating mice with TGCO at the doses of 40 and 80 mg/kg significantly antagonized reserpine-induced ptosis and hypothermia, suggesting that the antidepressant effect of TGCO may be mediated via central monoaminergic neurotransmitter system. | |||
TO cite this article:YANG Qiongxiong,HUANG Xiao-yan. The antidepressant-like effect of Cynanchum otophyllum[OL].[17 August 2011] http://en.paper.edu.cn/en_releasepaper/content/4439358 |
6. In-vitro Inhibitory Effect of Methanol Extracts of Chinese Herbal Drugs on Supercoiling Activity of Bacterial DNA Gyrase | |||
XU Hui,YAO Pan,LIN Guodong,CHEN Weiwen | |||
Traditional Chinese Medicine and Pharmacology 22 February 2011 | |||
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Abstract:A large number of Chinese herbal drugs (CHDs) exhibit antibacterial activities both in vivo and in vitro, but until now little is known regarding their inhibitory mechanisms. Bacterial DNA gyrase is a proven target for antibacterial agents. Aim of this study was to investigate the in-vitro inhibitory effect of methanol extracts of CHDs against supercoiling activity of bacterial DNA gyrase. Fifteen CHDs were selected and extracted with methanol, respectively. Inhibitory effect of the extracts on DNA gyrase was tested using gel-based DNA supercoiling assay. Among fifteen CHDs tested, methanol extracts of Lonicerae Japonicae Flos (S2), Taraxaci Herba (S7), Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (S8) demonstrated an obvious inhibitory effect against supercoiling activity of DNA gyrase, and the others were either less active or could not be determined with the present method. Moreover, it was likely that S7 and S8 inhibit gyrase in a concentration-dependent manner. In conclusion, DNA supercoiling assay is a promising method to study the inhibitory activity of CHDs on bacterial DNA gyrase. Some CHDs do have gyrase-inhibitory activity as proposed. Further investigations are needed to elucidate the inhibition mechanism of these CHDs on supercoiling activity of gyrase. | |||
TO cite this article:XU Hui,YAO Pan,LIN Guodong, et al. In-vitro Inhibitory Effect of Methanol Extracts of Chinese Herbal Drugs on Supercoiling Activity of Bacterial DNA Gyrase[OL].[22 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4411791 |
7. Factors Affecting Embryogenic Callus Production and Plant Regeneration from Anther Culture of Bupleurum chinense | |||
YANG Chengmin,ZHAO Yukai,WEI Jianhe,ZHAO Lizi,SUI Chun,Zhang Zhen,CUI Luying | |||
Traditional Chinese Medicine and Pharmacology 28 January 2011 | |||
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Abstract:To evaluate the influences of the genotypes, anther developmental stages and cultural conditions on the efficiency of embryogenic callus induction and plant regeneration in the anthers culture of Bupleurum chinense. Methods The different effects such as four genotypes, plant growth regulators, and temperature condition were compared in the experiments. Results The most embryogenic calli was achieved for the genotypes 'ZCYH', 'Z4' and 'Z5' at the early- to middle-uninucleate stages, for genotype 'ZPM1' at the tetrad stage. Cold pretreatment increased the production of the embryogenic callus, and 4-d cold pretreatment improved the production of embryogenic callus from 0 to 2.2% and 5.0% for genotypes 'ZPM1' and 'ZCYH', respectively. No embryogenic callus were induced in the medium containing less than 0.75 mg/L 2,4-D. The highest regeneration rate (34.6%) was obtained in the half-strength MS salts regeneration medium supplemented with BA 0.1 mg/L. The low concentration BA was able to promote the embryogenic callus formation and subsequent plantlet regeneration via somatic embryogenesis. Chromosome counting of regenerated plantlets showed mostly diploid plant (2n=12) with only one haploid plant (n=6). Because of the low rate of microspore embryo formation, we only track the process of embryogenesis from the connective tissue, instead of microspore by histological observations. Conclusion This study developed an efficient system embryogenic for callus induction and plant regeneration system from anthers of B. chinense. This is the first report on the haploid plantlet through the anther culture of B. chinense. | |||
TO cite this article:YANG Chengmin,ZHAO Yukai,WEI Jianhe, et al. Factors Affecting Embryogenic Callus Production and Plant Regeneration from Anther Culture of Bupleurum chinense[OL].[28 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4409604 |
8. Traditional Chinese Medicine | |||
Wang Chen,Lu Jinggen,Yin Haoqiang | |||
Traditional Chinese Medicine and Pharmacology 15 December 2010 | |||
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Abstract:Aim: This study was designed to develop and treat with traditional Chinese medicine method of an experimental rat model of subcutaneous fistula induced by bacteria infection. Methods: A spring-gauze was surgically implanted into 24 male Sprague-Dawley (SD) rat's dorsal muscle, respectively. Then Staphylococcus aureus and Escherichia Coli mixed bacteria were injected into the spring-gauze. After 45days, all spring-gauzes were removed under anesthesia. X-ray and ultrasound were performed, enabling imaging fistula track assessment. Every rat was examined by a fistula probe and four rats were randomly selected for histological fistula tract assessment at the same time. Residual rats were randomly divided into in two groups. Group A treated with traditional Chinese medical method "thread-dragging therapy". Group B treated with Western medical method "fistulotomy". The healing time and body weights of two groups were compared. Results: At 45days imaging assessment showed a track with two openings in all rats and histological results proven lumen and surrounding granulation tissue with epithelium similar to that criteria and features of fistula in human being. The mean healing time of group A was shorter than group B with significant differences. The body weight of group A is heavier than group B at 7days after surgery and 14days after surgery respectively. Conclusions: A rat model with subcutaneous fistula induced by Staphylococcus aureus and Escherichia Coli mixed bacteria infection is easy and repeatable. Traditional Chinese medicine therapy "thread-dragging therapy" is less invasive and safe. [Key words] Subcutaneous fistula; Infected model; Traditional Chinese medicine; Thread-dragging therapy | |||
TO cite this article:Wang Chen,Lu Jinggen,Yin Haoqiang. Traditional Chinese Medicine[OL].[15 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4397413 |
9. Preparation and Characterization of Resveratrol Molecularly Imprinted Polymeric Microspheres | |||
Hong Meihua,Chen Yuantao | |||
Traditional Chinese Medicine and Pharmacology 25 June 2010 | |||
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Abstract:The molecularly imprinted polymeric microspheres (MIPMs) have been successfully prepared by a single-step swelling and polymerization in polar solvent using the polystyrene particles as seeds, and resveratrol as template molecule. The resveratrol-MIPMs were characterized with scanning electronic microscope. The synthetic condition and the adsorption properties were also studied. It was found that the microspheres with concave-dipression structure had large surface area and lots of binding sites, effectively raising the combination rate and imprinted capacity. | |||
TO cite this article:Hong Meihua,Chen Yuantao. Preparation and Characterization of Resveratrol Molecularly Imprinted Polymeric Microspheres[OL].[25 June 2010] http://en.paper.edu.cn/en_releasepaper/content/4377209 |
10. Pharmacological effects on the composite Salvia miltiorrhiza combined with Acanthopanax senticosus injection of the Chinese herbal medicine preparation in rats with Coronary Atherosclerotic Heart Disease | |||
Xin Wang,Tingli Li,Yinfeng Tan | |||
Traditional Chinese Medicine and Pharmacology 24 November 2009 | |||
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Abstract:To investigate the mechanism of suppressive effect of Chinese medicine on Coronary Atherosclerotic Heart Disease (IHD) by observing the effect of Salvia miltiorrhiza injection coordinated with Acanthopanax senticosus injection(MSAI)on the acute myocardial ischemia rat. The optimal formula of MSAI was ascertained using design by uniform distribution. From pharmacodynamics, the rat was applied with isolated heart perfusion method, for measuring Coronary Artery Fluence . Further, Coronary Artery in rat was received physic liquor by intravenous injection using constant flow pump to determine left ventricular systolic pressure (LVSP) , left ventricular maximum pressure variation rate(LVMPVR)and left ventricular end-diastolic pressure(LVEDP). The MSAI was found that could significantly increase CF of the isolated heart of the rats(P<0.01),compared with SI and AI control group, respectively ; in addition,the effect of Formula (Ⅲ)(syringoside : protocatechuic aldehyde = 1:1.25)is the optimization. The results of hemodynamic effect of acute myocardial ischemia in 15min rat treated with MSAI demonstrated that MSAI could remarkably raise LVSP, LVMPVR(±dp/dtmax)and lower LVEDP. Additionally, the effect on resisting acute-myocardial-ischemia of MSAI group was better than that of SI group and AI group(P<0.01). The mechanism of suppressive effect of MSAI on IHD of may be realized by increasing CF, elevating LVSP, LVMPVR(±dp/dtmax),and lowering LVEDP. | |||
TO cite this article:Xin Wang,Tingli Li,Yinfeng Tan. Pharmacological effects on the composite Salvia miltiorrhiza combined with Acanthopanax senticosus injection of the Chinese herbal medicine preparation in rats with Coronary Atherosclerotic Heart Disease [OL].[24 November 2009] http://en.paper.edu.cn/en_releasepaper/content/36963 |
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